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Maintenance of genetic variation in plants and pathogens involves complex networks of gene-for-gene interactions

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Hall, Sharon A., Allen, Rebecca L., Baumber, Rachel E., Baxter, Laura, Fisher, Kate, Bittner-Eddy , Peter D., Rose, Laura E., Holub, E. B. and Beynon, Jim. (2009) Maintenance of genetic variation in plants and pathogens involves complex networks of gene-for-gene interactions. Molecular Plant Pathology, Vol.10 (No.4). pp. 449-457. ISSN 1464-6722

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Official URL: http://dx.doi.org/10.1111/j.1364-3703.2009.00544.x

Abstract

The RPP13 [recognition of Hyaloperonospora arabidopsidis (previously known as Peronospora parasitica)] resistance (R) gene in Arabidopsis thaliana exhibits the highest reported level of sequence diversity among known R genes. Consistent with a co-evolutionary model, the matching effector protein ATR13 (A. thaliana-recognized) from H. arabidopsidis reveals extreme levels of allelic diversity. We isolated 23 new RPP13 sequences from a UK metapopulation, giving a total of 47 when combined with previous studies. We used these in functional studies of the A. thaliana accessions for their resistance response to 16 isolates of H. arabidopsidis. We characterized the molecular basis of recognition by the expression of the corresponding ATR13 genes from these 16 isolates in these host accessions. This allowed the determination of which alleles of RPP13 were responsible for pathogen recognition and whether recognition was dependent on the RPP13/ATR13 combination. Linking our functional studies with phylogenetic analysis, we determined that: (i) the recognition of ATR13 is mediated by alleles in just a single RPP13 clade; (ii) RPP13 alleles in other clades have evolved the ability to detect other pathogen ATR protein(s); and (iii) at least one gene, unlinked to RPP13 in A. thaliana, detects a different subgroup of ATR13 alleles

Item Type:

Journal Article

Subjects:

S Agriculture > SB Plant culture
Q Science > QR Microbiology

Divisions:

Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)

Library of Congress Subject Headings (LCSH):

Arabidopsis thaliana, Plant genetics -- Technique, Plants -- Disease and pest resistance -- Genetic aspects

Journal or Publication Title:

Molecular Plant Pathology

Publisher:

Wiley-Blackwell Publishing Ltd.

ISSN:

1464-6722

Official Date:

20 March 2009

Dates:

Date	Event
20 March 2009	["eprint_fieldopt_dates_date_type_available" not defined]

Volume:

Vol.10

Number:

No.4

Page Range:

pp. 449-457

Identification Number:

10.1111/j.1364-3703.2009.00544.x

Status:

Peer Reviewed

Access rights to Published version:

Restricted or Subscription Access

References:

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