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Isolation and in vitro expansion of human colonic stem cells
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Jung, Peter, Sato, Toshiro, Merlos-Suárez, Anna, Barriga, Francisco M., Iglesias, Mar, Rossell, David, Auer, Herbert, Gallardo, Mercedes, Blasco, Maria A., Sancho, Elena, Clevers, Hans and Batlle, Eduard (2011) Isolation and in vitro expansion of human colonic stem cells. Nature Medicine, Volume 17 (Number 10). pp. 1225-1227. doi:10.1038/nm.2470 ISSN 1078-8956.
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Official URL: http://dx.doi.org/10.1038/nm.2470
Abstract
Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Statistics | ||||
Journal or Publication Title: | Nature Medicine | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 1078-8956 | ||||
Official Date: | 4 September 2011 | ||||
Dates: |
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Volume: | Volume 17 | ||||
Number: | Number 10 | ||||
Page Range: | pp. 1225-1227 | ||||
DOI: | 10.1038/nm.2470 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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