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A simple and fast protocol for the protein complex immunoprecipitation (Co-IP) of effector : host protein complexes
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Steinbrenner, Jens, Eldridge, Matthew, Tomé, Daniel F. A. and Beynon, Jim (2014) A simple and fast protocol for the protein complex immunoprecipitation (Co-IP) of effector : host protein complexes. In: Plant-Pathogen Interactions : Methods and Protocols. Methods in Molecular Biology, Volume 1127 . Berlin Heidelberg: Springer, pp. 195-211. ISBN 9781627039857
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Official URL: http://dx.doi.org/10.1007/978-1-62703-986-4_16
Abstract
Plant pathogens are responsible for enormous damage in natural and cultured ecosystems. One strategy most pathogenic organisms follow is the secretion of effector proteins that manipulate the host immune system to suppress defense responses. There is considerable interest in finding host targets of pathogen effectors as this helps to shape our understanding of how those proteins work in planta. The presented protocol describes a protein complex immunoprecipitation method aimed at verifying protein–protein interactions derived from protein complementation assays like Yeast-two-Hybrid.
| Item Type: | Book Item | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Series Name: | Methods in Molecular Biology | ||||
| Publisher: | Springer | ||||
| Place of Publication: | Berlin Heidelberg | ||||
| ISBN: | 9781627039857 | ||||
| ISSN: | 1064-3745 | ||||
| Book Title: | Plant-Pathogen Interactions : Methods and Protocols | ||||
| Official Date: | 2014 | ||||
| Dates: |
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| Volume: | Volume 1127 | ||||
| Page Range: | pp. 195-211 | ||||
| DOI: | 10.1007/978-1-62703-986-4_16 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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