Steinbrenner, Jens, Eldridge, Matthew, Tomé, Daniel F. A. and Beynon, Jim (2014) A simple and fast protocol for the protein complex immunoprecipitation (Co-IP) of effector : host protein complexes. In: Plant-Pathogen Interactions : Methods and Protocols. Methods in Molecular Biology, Volume 1127 . Berlin Heidelberg: Springer, pp. 195-211. ISBN 9781627039857

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Abstract

Plant pathogens are responsible for enormous damage in natural and cultured ecosystems. One strategy most pathogenic organisms follow is the secretion of effector proteins that manipulate the host immune system to suppress defense responses. There is considerable interest in finding host targets of pathogen effectors as this helps to shape our understanding of how those proteins work in planta. The presented protocol describes a protein complex immunoprecipitation method aimed at verifying protein–protein interactions derived from protein complementation assays like Yeast-two-Hybrid.

Item Type:	Book Item
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Series Name:	Methods in Molecular Biology
Publisher:	Springer
Place of Publication:	Berlin Heidelberg
ISBN:	9781627039857
ISSN:	1064-3745
Book Title:	Plant-Pathogen Interactions : Methods and Protocols
Official Date:	2014
Dates:	Date Event 2014 Published
Volume:	Volume 1127
Page Range:	pp. 195-211
DOI:	10.1007/978-1-62703-986-4_16
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access

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