The Library
Vitamin D reverses aPL-induced inflammation and LMWH-induced sFlt-1 release by human trophoblast
Tools
Tools
Tools
Gysler, Stefan M., Mulla, Melissa J., Stuhlman, Meredith, Sfakianaki, Anna K., Paidas, Michael J., Stanwood, Nancy L., Gariepy, Aileen, Brosens, Jan J., Chamley, Lawrence W. and Abrahams, Vikki M. (2014) Vitamin D reverses aPL-induced inflammation and LMWH-induced sFlt-1 release by human trophoblast. American Journal of Reproductive Immunology . n/a-n/a. doi:10.1111/aji.12301 ISSN 1046-7408.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1111/aji.12301
Abstract
Problem
Women with antiphospholipid syndrome (APS) are at increased risk of recurrent pregnancy loss (RPL) and preeclampsia. Antiphospholipid antibodies (aPL) directly alter trophoblast function. Treatment with low molecular weight heparin (LMWH) reduces the risk of RPL but not preeclampsia. Moreover, LMWH stimulates trophoblast sFlt-1 release, an anti-angiogenic factor associated with preeclampsia. Since vitamin D deficiency is associated with APS and preeclampsia, this study sought to determine the effect of vitamin D on trophoblast function in the setting of aPL and LMWH.
Method of study
A human first trimester trophoblast cell line (HTR8) and primary trophoblast cultures were treated with or without aPL in the presence and absence of vitamin D, LMWH or both. Trophoblast secretion of inflammatory cytokines and angiogenic factors were measured by ELISA.
Results
Vitamin D alone or in combination with LMWH attenuated the aPL-induced trophoblast inflammatory response in the HTR8 cells and primary cultures. While vitamin D did not have any impact on aPL-mediated modulation of angiogenic factors in the primary trophoblast, it significantly inhibited LMWH-induced sFlt-1 release.
Conclusion
LMWH in combination with vitamin D may be more beneficial than single-agent therapy by preventing aPL-induced trophoblast inflammation and reversing LMWH-induced sFlt-1 secretion.
| Item Type: | Journal Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Reproductive Health ( - until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||||||
| Journal or Publication Title: | American Journal of Reproductive Immunology | ||||||||
| Publisher: | Wiley-Blackwell Publishing, Inc. | ||||||||
| ISSN: | 1046-7408 | ||||||||
| Official Date: | 29 July 2014 | ||||||||
| Dates: |
|
||||||||
| Page Range: | n/a-n/a | ||||||||
| DOI: | 10.1111/aji.12301 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
