Novel use of stir bar sorptive extraction (SBSE) as a tool for isolation of oviposition site attractants for gravid Culex quinquefasciatus
Carson, Connor, Birkett, M. A., Logan, J. G., Mawa, K., Pates, H. V., Pickett, J. A. (John A.), Rwegoshora, R. T., Tungu, P. K. and Cameron, M. M.. (2010) Novel use of stir bar sorptive extraction (SBSE) as a tool for isolation of oviposition site attractants for gravid Culex quinquefasciatus. Bulletin of Entomological Research, Vol.100 (No.1). pp. 1-7. ISSN 0007-4853Full text not available from this repository.
Official URL: http://dx.doi.org/10.1017/S0007485309006701
Mosquitoes Such as Cidex quinquefasciaturs Say (Diptera: Culicidae) are important vectors of organisms that cause disease ill humans. Research into the development of effective standardized odour baits for blood-fed females (oviposition attractants), to enable entomological monitoring of vector Populations, is hampered by complex protocols for extraction of physiologically active volatile chemicals from natural breeding site water samples, which have produced inconsistent results. Air entrainment and solvent extraction are technically demanding methods and are impractical for use in resource poor environments where mosquito-borne disease is most prevalent. This study reports the first use of a simple, robust extraction technique, stir bar sorptive extraction (SBSE), to extract behaviourally active small lipophilic molecules (SLMs) present in water samples collected from Cx. quinquefasciatus breeding sites in Tanzania. Extracts from a pit latrine and from a cess pool breeding site attracted more gravid Cx. quinquefasciatus in pair choice bioassays than control extracts, and Coupled gas chromatography-electroantennography (GC-EAG) allowed tentative identification of 15 electrophysiologically active chemicals, including the known oviposition attractant, skatol (3-methyl-indole). Here, we have demonstrated, using simple pair choice bioassays in controlled laboratory conditions, that SBSE is effective for the extraction of behaviourally and electrophysiologically active semiochemicals from mosquito breeding site waters. Further research is required to confirm that SBSE is all appropriate technique for use in field surveys in the search for oviposition cues for Cx. quinquefasciatus.
|Item Type:||Journal Article|
|Subjects:||Q Science > QL Zoology|
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Bulletin of Entomological Research|
|Publisher:||Cambridge University Press|
|Number of Pages:||7|
|Page Range:||pp. 1-7|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)|
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