The Library
Characterization of vitamin D production by human ocular barrier cells
Tools
Tools
Tools
Alsalem, J. A., Patel, D., Susarla, R., Coca-Prados, M., Bland, Rosemary, Walker, E. A., Rauz, S. and Wallace, G. R. (2014) Characterization of vitamin D production by human ocular barrier cells. Investigative Ophthalmology & Visual Science, Volume 55 (Number 4). pp. 2140-2147. doi:10.1167/iovs.13-13019
Research output not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1167/iovs.13-13019
Abstract
Purpose. Vitamin D3 is a secosteroid mainly synthesized from the conversion of the skin precursor 7-dehydrocholesterol (7DHC) to vitamin D3 by ultraviolet (UV) B sunlight. Extrarenal synthesis of vitamin D3 has been reported in many tissues and cells, including barrier sites. This study characterizes the expression of components of vitamin D3 signaling in human ocular barrier cells.
Methods. Primary human scleral fibroblasts (HSF), human corneal endothelial (HCEC-12), nonpigmented ciliary body epithelial (ODM-2), and adult retinal pigment epithelial (ARPE-19) cell lines were analyzed for the expression of vitamin D receptor (VDR), the vitamin D3 activating enzymes 1α-hydroxylase (CYP27B1), 25-hydroxylases (CYP27A1 and CYP2R1), the vitamin D3 inactivating enzyme 24-hydroxylase (CYP24A1), and the endocytic receptors cubilin and megalin using a combination of RT-PCR, immunocytochemistry, and enzyme immunoassay (EIA).
Results. The HSF, HCEC-12, ODM-2, and ARPE-19 express mRNA and protein for all vitamin D3 synthesizing and metabolizing components. The cell types tested, except HSF, are able to convert inactive 25-hydroxyvitamin D3 (25[OH]D3) into active 1,25-hydroxyviatmin D3 (1,25[OH]2D3).
Conclusions. This novel study demonstrated that ocular barrier epithelial cells express the machinery for vitamin D3 and can produce 1,25(OH)2D3. We suggest that vitamin D3 might have a role in immune regulation and barrier function in ocular barrier epithelial cells.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Medicine > Warwick Medical School | ||||
| Journal or Publication Title: | Investigative Ophthalmology & Visual Science | ||||
| Publisher: | Association for Research in Vision and Ophthalmology | ||||
| ISSN: | 0146-0404 | ||||
| Official Date: | 7 April 2014 | ||||
| Dates: |
|
||||
| Volume: | Volume 55 | ||||
| Number: | Number 4 | ||||
| Page Range: | pp. 2140-2147 | ||||
| DOI: | 10.1167/iovs.13-13019 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
