Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Functional metagenomic analysis reveals rivers are a reservoir for diverse antibiotic resistance genes

Tools
- Tools
+ Tools

Amos, Gregory C. A., Zhang, Lihong, Hawkey, P. M., Gaze, William H. and Wellington, E. M. H. (2014) Functional metagenomic analysis reveals rivers are a reservoir for diverse antibiotic resistance genes. Veterinary Microbiology, Volume 171 (Number 3-4). pp. 441-447. doi:10.1016/j.vetmic.2014.02.017

[img]
Preview
PDF
WRAP_1-s2.0-S0378113514000947-main.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution.

Download (543Kb) | Preview
Official URL: http://dx.doi.org/10.1016/j.vetmic.2014.02.017

Request Changes to record.

Abstract

The environment harbours a significant diversity of uncultured bacteria and a potential source of novel and extant resistance genes which may recombine with clinically important bacteria disseminated into environmental reservoirs. There is evidence that pollution can select for resistance due to the aggregation of adaptive genes on mobile elements. The aim of this study was to establish the impact of waste water treatment plant (WWTP) effluent disposal to a river by using culture independent methods to study diversity of resistance genes downstream of the WWTP in comparison to upstream. Metagenomic libraries were constructed in Escherichia coli and screened for phenotypic resistance to amikacin, gentamicin, neomycin, ampicillin and ciprofloxacin. Resistance genes were identified by using transposon mutagenesis. A significant increase downstream of the WWTP was observed in the number of phenotypic resistant clones recovered in metagenomic libraries. Common β-lactamases such as blaTEM were recovered as well as a diverse range of acetyltransferases and unusual transporter genes, with evidence for newly emerging resistance mechanisms. The similarities of the predicted proteins to known sequences suggested origins of genes from a very diverse range of bacteria. The study suggests that waste water disposal increases the reservoir of resistance mechanisms in the environment either by addition of resistance genes or by input of agents selective for resistant phenotypes.

Item Type: Journal Article
Subjects: S Agriculture > S Agriculture (General)
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Sewage, Drug resistance in microorganisms
Journal or Publication Title: Veterinary Microbiology
Publisher: Elsevier
ISSN: 0378-1135
Official Date: 16 July 2014
Dates:
DateEvent
16 July 2014Published
16 February 2014Available
Volume: Volume 171
Number: Number 3-4
Number of Pages: 7
Page Range: pp. 441-447
DOI: 10.1016/j.vetmic.2014.02.017
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
Funder: Natural Environment Research Council (Great Britain) (NERC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), European Regional Development Fund (ERDF), European Science Foundation (ESF)
Grant number: NE/E004482/1 (NERC)

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us