Gutierrez-Caballero, Cristina, Burgess, Selena G., Bayliss, Richard and Royle, Stephen J. (2015) TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Biology Open, 4 . pp. 170-179. doi:10.1242/bio.201410843 ISSN 2046-6390.
Preview |
PDF (Creative Commons : Attribution 4.0)
WRAP_008359.1.full.pdf - Published Version - Requires a PDF viewer. Download (2MB) | Preview |
Abstract
The interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification.
Item Type: | Journal Article |
---|---|
Subjects: | Q Science > QR Microbiology |
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
Library of Congress Subject Headings (LCSH): | Microtubules, Rho-associated protein kinases |
Journal or Publication Title: | Biology Open |
Publisher: | The Company of Biologists Ltd. |
ISSN: | 2046-6390 |
Official Date: | 2015 |
Dates: | Date Event 2015 Published November 2014 Available 14 November 2014 Accepted 4 November 2014 Submitted |
Volume: | 4 |
Number of Pages: | 10 |
Page Range: | pp. 170-179 |
DOI: | 10.1242/bio.201410843 |
Status: | Peer Reviewed |
Publication Status: | Published |
Access rights to Published version: | Open Access (Creative Commons open licence) |
Date of first compliant deposit: | 28 July 2016 |
Date of first compliant Open Access: | 28 July 2016 |
Funder: | Cancer Research UK (CRUK), Warwick Medical School |
Grant number: | C25425/ A15182 (CRUK), C24461/A12772 (CRUK) |
URI: | https://wrap.warwick.ac.uk/65700/ |
Request changes or add full text files to a record
Repository staff actions (login required)
![]() |
View Item |