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TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation

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Gutierrez-Caballero, Cristina, Burgess, Selena G., Bayliss, Richard and Royle, Stephen J. (2015) TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Biology Open, 4 . pp. 170-179. doi:10.1242/bio.201410843

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Official URL: http://dx.doi.org/10.1242/bio.201410843

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Abstract

The interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Microtubules, Rho-associated protein kinases
Journal or Publication Title: Biology Open
Publisher: The Company of Biologists Ltd.
ISSN: 2046-6390
Official Date: 2015
Dates:
DateEvent
2015Published
November 2014Available
14 November 2014Accepted
4 November 2014Submitted
Volume: 4
Number of Pages: 10
Page Range: pp. 170-179
DOI: 10.1242/bio.201410843
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
Funder: Cancer Research UK (CRUK), Warwick Medical School
Grant number: C25425/ A15182 (CRUK), C24461/A12772 (CRUK)

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