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ALIX and ESCRT-III coordinately control cytokinetic abscission during germline stem cell division in vivo
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Eikenes, Åsmund H., Malerød, Lene, Christensen, Anette Lie, Steen, Chloé B., Mathieu, Juliette, Nezis, Ioannis P., Liestøl, Knut, Huynh, Jean-René, Stenmark, H. and Haglund, Kaisa (2015) ALIX and ESCRT-III coordinately control cytokinetic abscission during germline stem cell division in vivo. PLoS Genetics, Volume 11 (Number 1). pp. 1-7. Article number e1004904. doi:10.1371/journal.pgen.1004904
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Official URL: http://dx.doi.org/10.1371/journal.pgen.1004904
Abstract
Abscission is the final step of cytokinesis that involves the cleavage of the intercellular bridge connecting the two daughter cells. Recent studies have given novel insight into the spatiotemporal regulation and molecular mechanisms controlling abscission in cultured yeast and human cells. The mechanisms of abscission in living metazoan tissues are however not well understood. Here we show that ALIX and the ESCRT-III component Shrub are required for completion of abscission during Drosophila female germline stem cell (fGSC) division. Loss of ALIX or Shrub function in fGSCs leads to delayed abscission and the consequent formation of stem cysts in which chains of daughter cells remain interconnected to the fGSC via midbody rings and fusome. We demonstrate that ALIX and Shrub interact and that they co-localize at midbody rings and midbodies during cytokinetic abscission in fGSCs. Mechanistically, we show that the direct interaction between ALIX and Shrub is required to ensure cytokinesis completion with normal kinetics in fGSCs. We conclude that ALIX and ESCRT-III coordinately control abscission in Drosophila fGSCs and that their complex formation is required for accurate abscission timing in GSCs in vivo.
| Item Type: | Journal Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology | ||||||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||
| Library of Congress Subject Headings (LCSH): | Cytokinesis, Drosophila melanogaster, Abscission (Botany) | ||||||||
| Journal or Publication Title: | PLoS Genetics | ||||||||
| Publisher: | Public Library of Science | ||||||||
| ISSN: | 1553-7390 | ||||||||
| Official Date: | 30 January 2015 | ||||||||
| Dates: |
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| Volume: | Volume 11 | ||||||||
| Number: | Number 1 | ||||||||
| Number of Pages: | 33 | ||||||||
| Page Range: | pp. 1-7 | ||||||||
| Article Number: | Article number e1004904 | ||||||||
| DOI: | 10.1371/journal.pgen.1004904 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access | ||||||||
| Funder: | Norske kreftforening [Norwegian Cancer Society], European Research Council (ERC), South-Eastern Norway Regional Health Authority, Norges forskningsråd [Norwegian Research Council], European Molecular Biology Organization (EMBO) | ||||||||
| Grant number: | 605009 (NK), 233146 (ERC), 2012054 (SENRHA), 191569 (NRC), 179571 (NRC) |
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