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Deuterated carbohydrate probes as ‘label-free’ substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis

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Lowery, Richard, Gibson, Matthew I., Thompson, Richard C. and Fullam, Elizabeth (2015) Deuterated carbohydrate probes as ‘label-free’ substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis. Chemical communications, 51 (23). pp. 4838-4841. doi:10.1039/c4cc09588j ISSN 1359-7345.

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Official URL: http://dx.doi.org/10.1039/C4CC09588J

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Abstract

Understanding and probing small molecule uptake in cells is challenging, requiring sterically large chemical labels, or radioactive isotopes. Here, the uptake of deuterated sugars by Mycobacterium smegmatis, a non-pathogenic model of Mycobacterium tuberculosis, has been investigated using ion-beam (nuclear reaction) analysis demonstrating a new technique for label-free nutrient acquisition measurement.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Tuberculosis -- Microbiology
Journal or Publication Title: Chemical communications
Publisher: Royal Society of Chemistry
ISSN: 1359-7345
Official Date: 21 March 2015
Dates:
DateEvent
21 March 2015Published
12 February 2015Available
12 February 2015Accepted
1 December 2014Submitted
Volume: 51
Number: 23
Number of Pages: 4
Page Range: pp. 4838-4841
DOI: 10.1039/c4cc09588j
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 29 December 2015
Date of first compliant Open Access: 29 December 2015
Funder: Wellcome Trust (London, England), Advantage West Midlands (AWM), European Regional Development Fund (ERDF), Engineering and Physical Sciences Research Council (EPSRC), Birmingham Science City, Higher Education Funding Council for England (HEFCE), Royal Society (Great Britain)
Grant number: 104193/Z/14/Z (RS)

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