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Increased unsaturation of lipids in cytoplasmic lipid droplets in DAOY cancer cells in response to cisplatin treatment
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Pan, Xiaoyan, Wilson, Martin P., McConville, Carmel, Arvanitis, Theodoros N., Griffin, Julian L., Kauppinen, Risto A. and Peet, Andrew C. (2013) Increased unsaturation of lipids in cytoplasmic lipid droplets in DAOY cancer cells in response to cisplatin treatment. Metabolomics, Volume 9 (Number 3). pp. 722-729. doi:10.1007/s11306-012-0483-8 ISSN 1573-3882.
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Official URL: http://dx.doi.org/10.1007/s11306-012-0483-8
Abstract
ncreases in 1H nuclear magnetic resonance spectroscopy (NMR) visible lipids are a well-documented sign of treatment response in cancers. Lipids in cytoplasmic lipid droplets (LDs) are the main contributors to the NMR lipid signals. Two human primitive neuroectodermal tumour cell lines with different sensitivities to cisplatin treatment were studied. Increases in NMR visible saturated and unsaturated lipids in cisplatin treated DAOY cells were associated with the accumulation of LDs prior to DNA fragmentation due to apoptosis. An increase in unsaturated fatty acids (UFAs) was detected in isolated LDs from DAOY cells, in contrast to a slight decrease in UFAs in lipid extracts from whole cells. Oleic acid and linoleic acid were identified as the accumulating UFAs in LDs by heteronuclear single quantum coherence spectroscopy (HSQC). 1H NMR lipids in non-responding PFSK-1 cells were unchanged by exposure to 10 μM cisplatin. These findings support the potential of NMR detectable UFAs to serve as a non-invasive marker of tumour cell response to treatment.
Item Type: | Journal Article | ||||
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Subjects: | R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Engineering > WMG (Formerly the Warwick Manufacturing Group) | ||||
Library of Congress Subject Headings (LCSH): | Cancer cells, Cisplatin, Cancer -- Diagnosis, Nuclear magnetic resonance spectroscopy | ||||
Journal or Publication Title: | Metabolomics | ||||
Publisher: | Springer New York LLC | ||||
ISSN: | 1573-3882 | ||||
Official Date: | June 2013 | ||||
Dates: |
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Volume: | Volume 9 | ||||
Number: | Number 3 | ||||
Number of Pages: | 8 | ||||
Page Range: | pp. 722-729 | ||||
DOI: | 10.1007/s11306-012-0483-8 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 29 December 2015 | ||||
Date of first compliant Open Access: | 29 December 2015 | ||||
Funder: | Medical Research Council (Great Britain) (MRC), Andrew McCartney Trust Fund, Brain and Nervous System Tumour Research Fund, Birmingham Children's Hospital NHS Foundation Trust | ||||
Grant number: | G0601327 (MRC) |
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