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The size of cytoplasmic lipid droplets varies between tumour cell lines of the nervous system : a 1H NMR spectroscopy study
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Pan, Xiaoyan, Wilson, Martin P., McConville, Carmel, Arvanitis, Theodoros N., Kauppinen, Risto A. and Peet, Andrew C. (2012) The size of cytoplasmic lipid droplets varies between tumour cell lines of the nervous system : a 1H NMR spectroscopy study. Magnetic Resonance Materials in Physics, Biology and Medicine, Volume 25 (Number 6). pp. 479-485. doi:10.1007/s10334-012-0315-x
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Official URL: http://dx.doi.org/10.1007/s10334-012-0315-x
Abstract
Object:
Cytoplasmic lipid droplets (LDs) are dynamic cellular organelles; their accumulation is associated with several cellular processes, such as cell proliferation, apoptosis and necrosis. 1H Nuclear Magnetic Resonance (NMR) spectroscopy detects resonances from lipids present in cytoplasmic (LDs); an understanding of the relationship between LD characteristics and NMR lipid signals is important.
Materials and methods:
In this study, five nervous system cancer cell lines were investigated. Nile red staining was used to measure the diameter of LDs. High-resolution magic angle spinning NMR (HR-MAS) was performed on harvested cell pellets to quantify the patterns of lipid signals.
Results:
LDs were present in all five cell lines with different morphology. An average LD diameter of approximately 0.2 μm was found in all cell types. Diameter of the largest LDs varied across the cell lines. The intensity of NMR lipid signals varied greatly between cell types, and a good correlation was found between total volume of LDs and the proton NMR lipid signal intensity at 0.9 and 1.3 ppm.
Conclusion:
The correlation implied that little NMR signal is detected from LDs of diameters less than approximately 0.34 μm, most likely due to restriction of rotational motion of the lipids.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science > WMG (Formerly the Warwick Manufacturing Group) | ||||
| Journal or Publication Title: | Magnetic Resonance Materials in Physics, Biology and Medicine | ||||
| Publisher: | Springer-Verlag | ||||
| ISSN: | 0968-5243 | ||||
| Official Date: | December 2012 | ||||
| Dates: |
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| Volume: | Volume 25 | ||||
| Number: | Number 6 | ||||
| Page Range: | pp. 479-485 | ||||
| DOI: | 10.1007/s10334-012-0315-x | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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