Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

The lipid composition of isolated cytoplasmic lipid droplets from a human cancer cell line, BE(2)M17

Tools
- Tools
+ Tools

Pan, Xiaoyan, Wilson, Martin P., McConville, Carmel, Brundler, Marie-Anne, Arvanitis, Theodoros N., Shockcor, John P., Griffin, Julian L., Kauppinen, Risto A. and Peet, Andrew C. (2012) The lipid composition of isolated cytoplasmic lipid droplets from a human cancer cell line, BE(2)M17. Molecular BioSystems, Volume 8 (Number 6). pp. 1694-1700. doi:10.1039/c2mb05485j

Research output not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1039/C2MB05485J

Request Changes to record.

Abstract

1H nuclear magnetic resonance spectroscopy (NMR) resonances from lipids in tumours are associated with tumour grade and treatment response. The origin of these NMR signals is mainly considered to be cytoplasmic lipid droplets (LDs). Techniques exist for isolating LDs but little is known about their composition and its relationship to NMR signals. In this work, density-gradient ultracentrifugation was performed on homogenised human cancer cells to isolate LDs. 1H NMR was performed on whole cells, isolated LDs and their extracts. Heteronuclear single quantum coherence spectroscopy (HSQC) and liquid chromatography mass spectroscopy (LC-MS) were performed on lipid extracts of LDs. Staining and microscopy were used to characterize isolated LDs. An excellent agreement in chemical shift and relative signal intensity was observed between lipid resonances in cells and isolated LD spectra supporting that NMR-visible lipids originate primarily from LDs. Isolated LDs showed high concentrations of unsaturated lipids, a oleic-to-linoleic acid ratio greater than two and a cholesteryl ester (ChE)-to-cholesterol (Ch) ratio close to unity. These ratios were several-fold greater than respective ratios in whole cells, demonstrating isolation is important to characterize LD composition. LDs contain a specific group of lipid species that are likely to contribute to the 1H NMR spectrum of cells.

Item Type: Journal Article
Divisions: Faculty of Science > WMG (Formerly the Warwick Manufacturing Group)
Journal or Publication Title: Molecular BioSystems
Publisher: Royal Society of Chemistry
ISSN: 1742-206X
Official Date: 2012
Dates:
DateEvent
2012Published
Volume: Volume 8
Number: Number 6
Page Range: pp. 1694-1700
DOI: 10.1039/c2mb05485j
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us