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Active membrane transport and receptor proteins from bacteria
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UNSPECIFIED (2005) Active membrane transport and receptor proteins from bacteria. In: Conference on Mechanisms of Bioenergetic Membrane Proteins - Structures and Beyond, Wilhelm-Kempf Haus, Wiesbaden, GERMANY, MAR 20-24, 2005. Published in: BIOCHEMICAL SOCIETY TRANSACTIONS, 33 (Part 4). pp. 867-872. ISSN 0300-5127.
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Abstract
A general strategy for the expression of bacterial membrane transport and receptor genes in Escherichia coli is described. Expression is amplified so that the encoded proteins comprise 5-35% of E. coli inner membrane protein. Depending upon their topology, proteins are produced with RGSH(6) or a Strep tag at the C-terminus. These enable purification in mg quantities for crystallization and NMR studies. Examples of one nutrient uptake and one multidrug extrusion protein from Helicobacter pylori are described. This strategy is successful for membrane proteins from H. pylori, E. coli, Enterococcus faecalis, Bacillus subtilis, staphylococcus aureus, Microbacterium liquefaciens, Brucella abortus, Brucella melitensis, Campylobacter jejuni, Neisseria meningitides, Streptomyces coelicolor and Rhodobacter sphaeroides.
Item Type: | Conference Item (UNSPECIFIED) | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | BIOCHEMICAL SOCIETY TRANSACTIONS | ||||
Publisher: | PORTLAND PRESS LTD | ||||
ISSN: | 0300-5127 | ||||
Official Date: | August 2005 | ||||
Dates: |
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Volume: | 33 | ||||
Number: | Part 4 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 867-872 | ||||
Publication Status: | Published | ||||
Title of Event: | Conference on Mechanisms of Bioenergetic Membrane Proteins - Structures and Beyond | ||||
Location of Event: | Wilhelm-Kempf Haus, Wiesbaden, GERMANY | ||||
Date(s) of Event: | MAR 20-24, 2005 |
Data sourced from Thomson Reuters' Web of Knowledge
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