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A review of the behaviour and analysis of bolted connections and joints in pultruded fibre reinforced polymers
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Coelho, Ana M. Girão and Mottram, J. Toby (2015) A review of the behaviour and analysis of bolted connections and joints in pultruded fibre reinforced polymers. Materials & Design, 74 . pp. 86-107. doi:10.1016/j.matdes.2015.02.011 ISSN 0264-1275.
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Official URL: http://dx.doi.org/10.1016/j.matdes.2015.02.011
Abstract
Medicago truncatula is a model legume, whose genome is currently being sequenced. Somatic embryogenesis (SE) is a genotype-dependent character and not yet fully understood. In this study, a proteomic approach was used to compare the induction and expression phases of SE of both the highly embryogenic line M9-10a of M. truncatula cv. Jemalong and its non-embryogenic predecessor line, M9. The statistical analysis between the lines revealed 136 proteins with significant differential expression (P < 0.05). Of these, 5 had a presence/absence pattern in M9 vs M9-10a and 22 showed an at least twofold difference in terms of spot volume, were considered of particular relevance to the SE process and therefore chosen for identification. Spots were excised in gel digested with trypsin and proteins were identified using matrix-assisted laser desorption ionization-time of flight/time of flight. Identified proteins indicated a higher adaptability of the embryogenic line toward the stress imposed by the inducing culture conditions. Also, some proteins were shown to have a dual pattern of expression: peroxidase, pyrophosphatase and aspartate aminotransferase. These proteins showed higher expression during the induction phases of the M9 line, whereas in the embryogenic line had higher expression at stages coinciding with embryo formation.
| Item Type: | Journal Article | ||||||||||
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| Subjects: | T Technology > TA Engineering (General). Civil engineering (General) | ||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||||||
| Library of Congress Subject Headings (LCSH): | Fiber-reinforced plastics -- Joints | ||||||||||
| Journal or Publication Title: | Materials & Design | ||||||||||
| Publisher: | Elsevier Ltd | ||||||||||
| ISSN: | 0264-1275 | ||||||||||
| Official Date: | 5 June 2015 | ||||||||||
| Dates: |
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| Volume: | 74 | ||||||||||
| Number of Pages: | 22 | ||||||||||
| Page Range: | pp. 86-107 | ||||||||||
| DOI: | 10.1016/j.matdes.2015.02.011 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||
| Date of first compliant deposit: | 29 December 2015 | ||||||||||
| Date of first compliant Open Access: | 5 June 2017 | ||||||||||
| Funder: | Seventh Framework Programme (European Commission) (FP7) | ||||||||||
| Grant number: | PIEF-GA-2012-327142 |
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