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Presynaptic clathrin levels are a limiting factor for synaptic transmission

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Lopez-Murcia, F. J., Royle, Stephen J. and Llobet, A. (2014) Presynaptic clathrin levels are a limiting factor for synaptic transmission. Journal of Neuroscience, 34 (25). pp. 8618-8629. doi:10.1523/JNEUROSCI.5081-13.2014

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Official URL: http://dx.doi.org/10.1523/JNEUROSCI.5081-13.2014

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Abstract

To maintain communication, neurons must recycle their synaptic vesicles with high efficiency. This process places a huge burden on the clathrin-mediated endocytic machinery, but the consequences of this are poorly understood. We found that the amount of clathrin in a presynaptic terminal is not fixed. During stimulation, clathrin moves out of synapses as a function of stimulus strength and neurotransmitter release probability, which, together with membrane coat formation, transiently reduces the available pool of free clathrin triskelia. Correlative functional and morphological experiments in cholinergic autapses established by superior cervical ganglion neurons in culture show that presynaptic terminal function is compromised if clathrin levels fall by 20% after clathrin heavy chain knock down using RNAi. Synaptic transmission is depressed due to a reduction of cytoplasmic and readily releasable pools of vesicles. However, synaptic depression reverts after dialysis of exogenous clathrin, thus compensating RNAi-induced depletion. Lowering clathrin levels also reduces quantal size, which occurs concomitantly with a decrease in the size of synaptic vesicles. Large dense-core vesicles are unaffected by clathrin knock down. Together, our results show that clathrin levels are a dynamic property of presynaptic terminals that can influence short-term plasticity in a stimulus-dependent manner.

Item Type: Journal Article
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Medicine > Warwick Medical School
Journal or Publication Title: Journal of Neuroscience
Publisher: Society for Neuroscience
ISSN: 0270-6474
Official Date: 18 June 2014
Dates:
DateEvent
18 June 2014Published
22 May 2014Accepted
5 December 2013Submitted
Volume: 34
Number: 25
Page Range: pp. 8618-8629
DOI: 10.1523/JNEUROSCI.5081-13.2014
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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