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Extra-nuclear telomerase reverse transcriptase (TERT) regulates glucose transport in skeletal muscle cells
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Shaheen, Fozia, Grammatopoulos, Dimitris, Müller, Jürgen, Zammit, Victor A. and Lehnert, Hendrik (2014) Extra-nuclear telomerase reverse transcriptase (TERT) regulates glucose transport in skeletal muscle cells. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Volume 1842 (Number 9). pp. 1762-1769. doi:10.1016/j.bbadis.2014.06.018 ISSN 0925-4439.
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Official URL: http://dx.doi.org/10.1016/j.bbadis.2014.06.018
Abstract
Telomerase reverse transcriptase (TERT) is a key component of the telomerase complex. By lengthening telomeres in DNA strands, TERT increases senescent cell lifespan. Mice that lack TERT age much faster and exhibit age-related conditions such as osteoporosis, diabetes and neurodegeneration. Accelerated telomere shortening in both human and animal models has been documented in conditions associated with insulin resistance, including T2DM. We investigated the role of TERT, in regulating cellular glucose utilisation by using the myoblastoma cell line C2C12, as well as primary mouse and human skeletal muscle cells. Inhibition of TERT expression or activity by using siRNA (100 nM) or specific inhibitors (100 nM) reduced basal 2-deoxyglucose uptake by ~ 50%, in all cell types, without altering insulin responsiveness. In contrast, TERT over-expression increased glucose uptake by 3.25-fold. In C2C12 cells TERT protein was mostly localised intracellularly and stimulation of cells with insulin induced translocation to the plasma membrane. Furthermore, co-immunoprecipitation experiments in C2C12 cells showed that TERT was constitutively associated with glucose transporters (GLUTs) 1, 4 and 12 via an insulin insensitive interaction that also did not require intact PI3-K and mTOR pathways. Collectively, these findings identified a novel extra-nuclear function of TERT that regulates an insulin-insensitive pathway involved in glucose uptake in human and mouse skeletal muscle cells.
| Item Type: | Journal Article | ||||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | ||||||||
| Publisher: | Elsevier BV | ||||||||
| ISSN: | 0925-4439 | ||||||||
| Official Date: | 23 June 2014 | ||||||||
| Dates: |
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| Volume: | Volume 1842 | ||||||||
| Number: | Number 9 | ||||||||
| Page Range: | pp. 1762-1769 | ||||||||
| DOI: | 10.1016/j.bbadis.2014.06.018 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access |
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