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A novel isoform of MAP4 organises the paraxial microtubule array required for muscle cell differentiation
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Mogessie, Binyam, Roth, Daniel, Rahil, Zainab and Straube, Anne (2015) A novel isoform of MAP4 organises the paraxial microtubule array required for muscle cell differentiation. eLife, Volume 4 . e05697. doi:10.7554/eLife.05697 ISSN 2050-084X.
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Official URL: http://dx.doi.org/10.7554/eLife.05697
Abstract
The microtubule cytoskeleton is critical for muscle cell differentiation and undergoes reorganisation into an array of paraxial microtubules, which serves as template for contractile sarcomere formation. Here, we identify a previously uncharacterised isoform of microtubule-associated protein MAP4, oMAP4, as a microtubule organising factor that is crucial for myogenesis. We show that oMAP4 is expressed upon muscle cell differentiation and is the only MAP4 isoform essential for normal progression of the myogenic differentiation programme. Depletion of oMAP4 impairs cell elongation and cell-cell fusion. Most notably, oMAP4 is required for paraxial microtubule organisation in muscle cells and prevents dynein- and kinesin-driven microtubule-microtubule sliding. Purified oMAP4 aligns dynamic microtubules into antiparallel bundles that withstand motor forces in vitro. We propose a model in which the cooperation of dynein-mediated microtubule transport and oMAP4-mediated zippering of microtubules drives formation of a paraxial microtubule array that provides critical support for the polarisation and elongation of myotubes.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QM Human anatomy | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Cytoskeleton -- Microbiology | ||||||||
| Journal or Publication Title: | eLife | ||||||||
| Publisher: | eLife Sciences Publications Ltd. | ||||||||
| ISSN: | 2050-084X | ||||||||
| Official Date: | 21 April 2015 | ||||||||
| Dates: |
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| Volume: | Volume 4 | ||||||||
| Number of Pages: | 44 | ||||||||
| Article Number: | e05697 | ||||||||
| DOI: | 10.7554/eLife.05697 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 29 December 2015 | ||||||||
| Date of first compliant Open Access: | 29 December 2015 | ||||||||
| Funder: | Marie Curie Cancer Care, University of Warwick, Lister Institute of Preventive Medicine |
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