Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

A novel isoform of MAP4 organises the paraxial microtubule array required for muscle cell differentiation

Tools
- Tools
+ Tools

Mogessie, Binyam, Roth, Daniel, Rahil, Zainab and Straube, Anne (2015) A novel isoform of MAP4 organises the paraxial microtubule array required for muscle cell differentiation. eLife, Volume 4 . e05697. doi:10.7554/eLife.05697 ISSN 2050-084X.

[img]
Preview
PDF (Creative Commons : Attribution 4.0)
WRAP_eLife.05697.full.pdf - Published Version - Requires a PDF viewer.

Download (4Mb) | Preview
Official URL: http://dx.doi.org/10.7554/eLife.05697

Request Changes to record.

Abstract

The microtubule cytoskeleton is critical for muscle cell differentiation and undergoes reorganisation into an array of paraxial microtubules, which serves as template for contractile sarcomere formation. Here, we identify a previously uncharacterised isoform of microtubule-associated protein MAP4, oMAP4, as a microtubule organising factor that is crucial for myogenesis. We show that oMAP4 is expressed upon muscle cell differentiation and is the only MAP4 isoform essential for normal progression of the myogenic differentiation programme. Depletion of oMAP4 impairs cell elongation and cell-cell fusion. Most notably, oMAP4 is required for paraxial microtubule organisation in muscle cells and prevents dynein- and kinesin-driven microtubule-microtubule sliding. Purified oMAP4 aligns dynamic microtubules into antiparallel bundles that withstand motor forces in vitro. We propose a model in which the cooperation of dynein-mediated microtubule transport and oMAP4-mediated zippering of microtubules drives formation of a paraxial microtubule array that provides critical support for the polarisation and elongation of myotubes.

Item Type: Journal Article
Subjects: Q Science > QM Human anatomy
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Cytoskeleton -- Microbiology
Journal or Publication Title: eLife
Publisher: eLife Sciences Publications Ltd.
ISSN: 2050-084X
Official Date: 21 April 2015
Dates:
DateEvent
21 April 2015Published
19 April 2015Accepted
20 November 2014Submitted
Volume: Volume 4
Number of Pages: 44
Article Number: e05697
DOI: 10.7554/eLife.05697
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 29 December 2015
Date of first compliant Open Access: 29 December 2015
Funder: Marie Curie Cancer Care, University of Warwick, Lister Institute of Preventive Medicine

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us