Maier, Elisabeth, Wirnsberger, Gerald, Horejs-Hoeck, Jutta, Duschl, Albert and Hebenstreit, Daniel (2007) Identification of a distal tandem STAT6 element within the CCL17 locus. Human Immunology, Volume 68 (Number 12). pp. 986-992. doi:10.1016/j.humimm.2007.10.012

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Abstract

The CC chemokine ligand CCL17 is one of the major chemo-attractants for TH2 cells. Interleukin-4–induced activation of CCL17 expression was recently demonstrated to result from two STAT6 motifs in the proximal promoter. Here we provide evidence that a distal tandem STAT6 element further elevates expression from the CCL17 locus approximately twofold. This is demonstrated by reporter gene assays using different fragments of the CCL17 promoter and the region 2.5 kb upstream from the transcriptional start site. By electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation experiments, we show that STAT6 binds to the motifs in vitro and in vivo, respectively. Insertion of nucleotide exchanges into the STAT6 core motifs results in diminished promoter activation and abrogated STAT6 binding, as demonstrated by reporter gene and EMSA studies. Collectively these data reveal an additional element involved in the regulation of CCL17 expression.

Item Type:	Journal Article
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title:	Human Immunology
Publisher:	Elsevier Science BV
ISSN:	0198-8859
Official Date:	December 2007
Dates:	Date Event December 2007 Published
Volume:	Volume 68
Number:	Number 12
Page Range:	pp. 986-992
DOI:	10.1016/j.humimm.2007.10.012
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access

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