The Library
Identification of major zinc-binding proteins from a marine cyanobacterium : insight into metal uptake in oligotrophic environments
Tools
Barnett, James P., Scanlan, David J. and Blindauer, Claudia A. (2014) Identification of major zinc-binding proteins from a marine cyanobacterium : insight into metal uptake in oligotrophic environments. Metallomics, Volume 6 (Number 7). pp. 1254-1268. doi:10.1039/c4mt00048j ISSN 1756-5901.
|
PDF
WRAP_c4mt00048j.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution Non-commercial. Download (3246Kb) | Preview |
Official URL: http://dx.doi.org/10.1039/C4MT00048J
Abstract
Marine cyanobacteria make a significant contribution to primary production whilst occupying some of the most nutrient poor regions of the world's oceans. The low bioavailability of trace metals can limit the growth of phytoplankton in ocean waters, but only scarce data are available on the requirements of marine microbes for zinc. Recent genome mining studies suggest that marine cyanobacteria have both uptake systems for zinc and proteins that utilize zinc as a cofactor. In this study, the oligotrophic strain Synechococcus sp. WH8102 was grown at different zinc concentrations. Using metalloproteomics approaches, we demonstrate that even though this organism's growth was not affected by extremely low zinc levels, cells accumulated significant quantities of zinc, which was shown to be protein-associated by 2D liquid chromatography and ICP-MS. This indicates that the mechanisms for zinc uptake in Synechococcus sp. WH8102 are extremely efficient. Significantly, expression of SYNW2224, a putative porin, was up-regulated during growth in zinc-depleted conditions. Furthermore, along with 30 other proteins, SYNW2224 was captured by immobilised zinc affinity chromatography, indicating the presence of surface-exposed site(s) with metal-binding capacity. It is proposed that this porin plays a role in high-affinity zinc uptake in this and other cyanobacteria.
Item Type: | Journal Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QL Zoology | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
||||||||
Library of Congress Subject Headings (LCSH): | Cyanobacterial blooms -- Monitoring, Zinc -- Physiological effect | ||||||||
Journal or Publication Title: | Metallomics | ||||||||
Publisher: | Royal Society of Chemistry | ||||||||
ISSN: | 1756-5901 | ||||||||
Official Date: | 1 July 2014 | ||||||||
Dates: |
|
||||||||
Volume: | Volume 6 | ||||||||
Number: | Number 7 | ||||||||
Number of Pages: | 15 | ||||||||
Page Range: | pp. 1254-1268 | ||||||||
DOI: | 10.1039/c4mt00048j | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 29 December 2015 | ||||||||
Date of first compliant Open Access: | 29 December 2015 | ||||||||
Funder: | Leverhulme Trust (LT), Natural Environment Research Council (Great Britain) (NERC), Birmingham Science City, Advantage West Midlands (AWM), European Regional Development Fund (ERDF) | ||||||||
Grant number: | F/00 215/AY (LT), NE/F004249/1 (NERC) | ||||||||
Adapted As: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year