Wright, Jordan L., Atwan, Zeenah, Morris, S. J. (Susan J.) and Leppard, Keith (2015) The human adenovirus type 5 L4 promoter is negatively regulated by TFII-I and by L4-33K. Journal of Virology, Volume 89 (Number 14). pp. 1-36. doi:10.1128/JVI.00683-15

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Abstract

The late phase of adenovirus gene expression is controlled by proteins made in the intermediate phase, including L4 22K and 33K proteins that are expressed initially from the L4 promoter (L4P). L4P is activated by a combination of viral proteins and cellular p53 and is ultimately inhibited again by its own products. Here we have examined L4P of human adenovirus type 5 in detail and have defined its transcription start site, which our data suggest is positioned by a weak TATA box. Rather than contributing positively to promoter activity, a putative initiator element at the transcription start site acts as a target for negative regulation imposed on L4P by cellular TFII-I. We show that this TFII-I inhibition is relieved by one of the previously defined viral activators of L4P, E4 Orf3 protein, which alters the pool of TFII-I in the cell. We also explore further the negative regulation of L4P by its products, and show that the L4-33K protein is more significant in this process than L4-22K. It is the combined action of positive and negative factors that leads to the transient activation of L4P at the onset of the late phase of adenovirus gene expression.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology > QR355 Virology
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Adenoviruses, Genetic transcription, Gene expression
Journal or Publication Title:	Journal of Virology
Publisher:	American Society for Microbiology
ISSN:	0022-538X
Official Date:	July 2015
Dates:	Date Event July 2015 Published 29 April 2015 Available 20 April 2015 Accepted 12 March 2015 Submitted
Volume:	Volume 89
Number:	Number 14
Number of Pages:	36
Page Range:	pp. 1-36
DOI:	10.1128/JVI.00683-15
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Wellcome Trust (London, England)
Grant number:	WT094713MA (WT)
Open Access Version:	Publisher

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