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Tuning LeSPL-CNR expression by SlymiR157 affects tomato fruit ripening
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(2015) Tuning LeSPL-CNR expression by SlymiR157 affects tomato fruit ripening. Scientific Reports, Volume 5 . pp. 1-6. Article number 7852. doi:10.1038/srep07852 ISSN 2045-2322.
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Official URL: http://dx.doi.org/10.1038/srep07852
Abstract
In plants, microRNAs (miRNAs) play essential roles in growth, development, yield, stress response and interactions with pathogens. However no miRNA has been experimentally documented to be functionally involved in fruit ripening although many miRNAs have been profiled in fruits. Here we show that SlymiR157 and SlymiR156 differentially modulate ripening and softening in tomato (Solanum lycopersicum). SlymiR157 is expressed and developmentally regulated in normal tomato fruits and in those of the Colourless non-ripening (Cnr) epimutant. It regulates expression of the key ripening gene LeSPL-CNR in a likely dose-dependent manner through miRNA-induced mRNA degradation and translation repression. Viral delivery of either pre-SlymiR157 or mature SlymiR157 results in delayed ripening. Furthermore, qRT-PCR profiling of key ripening regulatory genes indicates that the SlymiR157-target LeSPL-CNR may affect expression of LeMADS-RIN, LeHB1, SlAP2a and SlTAGL1. However SlymiR156 does not affect the onset of ripening, but it impacts fruit softening after the red ripe stage. Our findings reveal that working together with a ripening network of transcription factors, SlymiR157 and SlymiR156 form a critical additional layer of regulatory control over the fruit ripening process in tomato.
Item Type: | Journal Article | ||||||||
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Subjects: | S Agriculture > SB Plant culture | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Clinical Trials Unit Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Tomatoes -- Genetics, Tomatoes -- Ripening | ||||||||
Journal or Publication Title: | Scientific Reports | ||||||||
Publisher: | Nature Publishing Group | ||||||||
ISSN: | 2045-2322 | ||||||||
Official Date: | 19 January 2015 | ||||||||
Dates: |
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Volume: | Volume 5 | ||||||||
Number of Pages: | 6 | ||||||||
Page Range: | pp. 1-6 | ||||||||
Article Number: | Article number 7852 | ||||||||
DOI: | 10.1038/srep07852 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 29 December 2015 | ||||||||
Date of first compliant Open Access: | 29 December 2015 | ||||||||
Funder: | Hangzhou shi fan da xue, Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Royal Society (Great Britain), Guo jia zi ran ke xue ji jin wei yuan hui (China) [National Natural Science Foundation of China] (NSFC) | ||||||||
Grant number: | 201108 (HSFDX), BBS/E/H/00YH0271 (BBSRC), 2007/R1 (RC), 31370180 (NSFC), 31401926 (NSFC), 31200913 (NSFC), 31201490 (NSFC), LQ13C020004 (NSFC), LQ13C060003 (NSFC), LQ12C02005 (NSFC), LY14C010005 (NSFC) |
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