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Seed dormancy cycling in Arabidopsis : chromatin remodelling and regulation of DOG1 in response to seasonal environmental signals
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Footitt, Steven, Müller, Kerstin, Kermode, Allison R. and Finch-Savage, William E. (2015) Seed dormancy cycling in Arabidopsis : chromatin remodelling and regulation of DOG1 in response to seasonal environmental signals. The plant journal, Volume 81 (Number 3). pp. 413-425. doi:10.1111/tpj.12735
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Official URL: http://dx.doi.org/10.1111/tpj.12735
Abstract
The involvement of chromatin remodelling in dormancy cycling in the soil seed bank (SSB) is poorly understood. Natural variation between the winter and summer annual Arabidopsis ecotypes Cvi and Bur was exploited to investigate the expression of genes involved in chromatin remodelling via histone 2B (H2B) ubiquitination/de-ubiquitination and histone acetylation/deacetylation, the repressive histone methyl transferases CURLY LEAF (CLF) and SWINGER (SWN), and the gene silencing repressor ROS1 (REPRESSOR OF SILENCING1) and promoter of silencing KYP/SUVH4 (KRYPTONITE), during dormancy cycling in the SSB. ROS1 expression was positively correlated with dormancy while the reverse was observed for CLF and KYP/SUVH4. We propose ROS1 dependent repression of silencing and a sequential requirement of CLF and KYP/SUVH4 dependent gene repression and silencing for the maintenance and suppression of dormancy during dormancy cycling. Seasonal expression of H2B modifying genes was correlated negatively with temperature and positively with DOG1 expression, as were histone acetyltransferase genes, with histone deacetylases positively correlated with temperature. Changes in the histone marks H3K4me3 and H3K27me3 were seen on DOG1 (DELAY OF GERMINATION1) in Cvi during dormancy cycling. H3K4me3 activating marks remained stable along DOG1. During relief of dormancy, H3K27me3 repressive marks slowly accumulated and accelerated on exposure to light completing dormancy loss. We propose that these marks on DOG1 serve as a thermal sensing mechanism during dormancy cycling in preparation for light repression of dormancy. Overall, chromatin remodelling plays a vital role in temporal sensing through regulation of gene expression.
| Item Type: | Journal Article | ||||||||||
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| Subjects: | S Agriculture > SB Plant culture | ||||||||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||||
| Library of Congress Subject Headings (LCSH): | Arabidopsis -- Seeds -- Dormancy | ||||||||||
| Journal or Publication Title: | The plant journal | ||||||||||
| Publisher: | Wiley-Blackwell | ||||||||||
| ISSN: | 0960-7412 | ||||||||||
| Official Date: | February 2015 | ||||||||||
| Dates: |
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| Date of first compliant deposit: | 29 December 2015 | ||||||||||
| Volume: | Volume 81 | ||||||||||
| Number: | Number 3 | ||||||||||
| Number of Pages: | 13 | ||||||||||
| Page Range: | pp. 413-425 | ||||||||||
| DOI: | 10.1111/tpj.12735 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Open Access | ||||||||||
| Funder: | Great Britain. Department for Environment, Food and Rural Affairs (DEFRA), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Marie Curie International Outgoing Fellowship (IOF) | ||||||||||
| Grant number: | BB/ I022201/1 (BBSRC) |
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