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Increased DNA dicarbonyl glycation and oxidation markers in patients with type 2 diabetes and link to diabetic nephropathy
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Waris, Sahar, Winklhofer-Roob, Brigitte M., Roob, Johannes M., Fuchs, Sebastian W., Sourij, Harald, Rabbani, Naila and Thornalley, Paul J. (2015) Increased DNA dicarbonyl glycation and oxidation markers in patients with type 2 diabetes and link to diabetic nephropathy. Journal of diabetes research, 2015 . pp. 1-10. 915486. doi:10.1155/2015/915486 ISSN 2314-6745.
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Official URL: http://dx.doi.org/10.1155/2015/915486
Abstract
Aim.
The aim of this study was to assess the changes of markers of DNA damage by glycation and oxidation in patients with type 2 diabetes and the association with diabetic nephropathy.
Methodology.
DNA oxidation and glycation adducts were analysed in plasma and urine by stable isotopic dilution analysis liquid chromatography-tandem mass spectrometry. DNA markers analysed were as follows: the oxidation adduct 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-OxodG) and glycation adducts of glyoxal and methylglyoxal—imidazopurinones GdG, MGdG, and N2-(1,R/S-carboxyethyl)deoxyguanosine (CEdG).
Results. Plasma 8-OxodG and GdG were increased 2-fold and 6-fold, respectively, in patients with type 2 diabetes, with respect to healthy volunteers. Median urinary excretion rates of 8-OxodG, GdG, MGdG, and CEdG were increased 28-fold, 10-fold, 2-fold, and 2-fold, respectively, in patients with type 2 diabetes with respect to healthy controls. In patients with type 2 diabetes, nephropathy was associated with increased plasma 8-OxodG and increased urinary GdG and CEdG. In a multiple logistic regression model for diabetic nephropathy, diabetic nephropathy was linked to systolic blood pressure and urinary CEdG.
Conclusion.
DNA oxidative and glycation damage-derived nucleoside adducts are increased in plasma and urine of patients with type 2 diabetes and further increased in patients with diabetic nephropathy.
| Item Type: | Journal Article | ||||||||
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| Subjects: | R Medicine > RC Internal medicine | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Diabetic nephropathies, Diabetes -- Complications | ||||||||
| Journal or Publication Title: | Journal of diabetes research | ||||||||
| Publisher: | Hindawi Publishing Corporation | ||||||||
| ISSN: | 2314-6745 | ||||||||
| Official Date: | 2015 | ||||||||
| Dates: |
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| Volume: | 2015 | ||||||||
| Number of Pages: | 10 | ||||||||
| Page Range: | pp. 1-10 | ||||||||
| Article Number: | 915486 | ||||||||
| DOI: | 10.1155/2015/915486 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 29 December 2015 | ||||||||
| Date of first compliant Open Access: | 29 December 2015 | ||||||||
| Funder: | Commonwealth Scholarship Commission in the United Kingdom (CSCUK), Sixth Framework Programme (European Commission) (FP6), Fifth Framework Programme (European Commission) (FP5) | ||||||||
| Grant number: | LSHM-CT-2005-018733, PREDICTIONS (FP6), QLK1-CT-1999-00830, VITAGE (FP5) |
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