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A novel collagen gel-based measurement technique for quantitation of cell contraction force

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Jin, Tianrong, Li, L., Siow, R. C. M. and Liu, Kuo-Kang (2015) A novel collagen gel-based measurement technique for quantitation of cell contraction force. Journal of The Royal Society Interface, Volume 12 (Number 106). Article number 20141365. doi:10.1098/rsif.2014.1365

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Official URL: http://dx.doi.org/10.1098/rsif.2014.1365

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Abstract

Cell contraction force plays an important role in wound healing, inflammation, angiogenesis and metastasis. This study describes a novel method to quantify single cell contraction force in vitro using human aortic adventitial fibroblasts embedded in a collagen gel. The technique is based on a depth sensing nano-indentation tester to measure the thickness and elasticity of collagen gels containing stimulated fibroblasts and a microscopy imaging system to estimate the gel area. In parallel, a simple theoretical model has been developed to calculate cell contraction force based on the measured parameters. Histamine (100 µM) was used to stimulate fibroblast contraction while the myosin light chain kinase inhibitor ML-7 (25 µM) was used to inhibit cell contraction. The collagen matrix used in the model provides a physiological environment for fibroblast contraction studies. Measurement of changes in collagen gel elasticity and thickness arising from histamine treatments provides a novel convenient technique to measure cell contraction force within a collagen matrix. This study demonstrates that histamine can elicit a significant increase in contraction force of fibroblasts embedded in collagen, while the Young's modulus of the gel decreases due to the gel degradation.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Engineering
Library of Congress Subject Headings (LCSH): Cytology—Research
Journal or Publication Title: Journal of The Royal Society Interface
Publisher: The Royal Society Publishing
ISSN: 1742-5689
Official Date: 8 April 2015
Dates:
DateEvent
8 April 2015Published
16 March 2015Accepted
12 December 2014Submitted
Volume: Volume 12
Number: Number 106
Article Number: Article number 20141365
DOI: 10.1098/rsif.2014.1365
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Leverhulme Trust (LT), British Heart Foundation
Grant number: PRG-2012-738; FS/09/037/27827;

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