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How blebs and pseudopods cooperate during chemotaxis
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Tyson, Richard A. (Richard Anthony), Zatulovskiy, Evgeny, Kay, Robert R. and Bretschneider, Till (2014) How blebs and pseudopods cooperate during chemotaxis. Proceedings of the National Academy of Sciences of the United States of America, 111 (32). pp. 11703-11708. doi:10.1073/pnas.1322291111
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Official URL: http://dx.doi.org/10.1073/pnas.1322291111
Abstract
Two motors can drive extension of the leading edge of motile cells: actin polymerization and myosin-driven contraction of the cortex, producing fluid pressure and the formation of blebs. Dictyostelium cells can move with both blebs and actin-driven pseudopods at the same time, and blebs, like pseudopods, can be orientated by chemotactic gradients. Here we ask how bleb sites are selected and how the two forms of projection cooperate. We show that membrane curvature is an important, yet overlooked, factor. Dictyostelium cells were observed moving under agarose, which efficiently induces blebbing, and the dynamics of membrane deformations were analyzed. Blebs preferentially originate from negatively curved regions, generated on the flanks of either extending pseudopods or blebs themselves. This is true of cells at different developmental stages, chemotaxing to either folate or cyclic AMP and moving with both blebs and pseudopods or with blebs only. A physical model of blebbing suggests that detachment of the cell membrane is facilitated in concave areas of the cell, where membrane tension produces an outward directed force, as opposed to pulling inward in convex regions. Our findings assign a role to membrane tension in spatially coupling blebs and pseudopods, thus contributing to clustering protrusions to the cell front.
| Item Type: | Journal Article | ||||||
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| Subjects: | Q Science > QH Natural history Q Science > QR Microbiology |
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| Divisions: | Faculty of Science > Computer Science Faculty of Science > Centre for Systems Biology |
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| Library of Congress Subject Headings (LCSH): | Chemotaxis, Cell membranes, Blisters, Cytoskeleton | ||||||
| Journal or Publication Title: | Proceedings of the National Academy of Sciences of the United States of America | ||||||
| Publisher: | National Academy of Sciences | ||||||
| ISSN: | 0027-8424 | ||||||
| Official Date: | 12 August 2014 | ||||||
| Dates: |
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| Date of first compliant deposit: | 11 October 2016 | ||||||
| Volume: | 111 | ||||||
| Number: | 32 | ||||||
| Page Range: | pp. 11703-11708 | ||||||
| DOI: | 10.1073/pnas.1322291111 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||
| Embodied As: | 1 |
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