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Bleb-driven chemotaxis of Dictyostelium cells
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Zatulovskiy, Evgeny, Tyson, Richard A. (Richard Anthony), Bretschneider, Till and Kay, Robert R. (2014) Bleb-driven chemotaxis of Dictyostelium cells. The Journal of Cell Biology, 204 (6). pp. 1027-1044. doi:10.1083/jcb.201306147 ISSN 0021-9525.
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Official URL: http://doi.org/10.1083/jcb.201306147
Abstract
Blebs and F-actin-driven pseudopods are alternative ways of extending the leading edge of migrating cells. We show that Dictyostelium cells switch from using predominantly pseudopods to blebs when migrating under agarose overlays of increasing stiffness. Blebs expand faster than pseudopods leaving behind F-actin scars, but are less persistent. Blebbing cells are strongly chemotactic to cyclic-AMP, producing nearly all of their blebs up-gradient. When cells re-orientate to a needle releasing cyclic-AMP, they stereotypically produce first microspikes, then blebs and pseudopods only later. Genetically, blebbing requires myosin-II and increases when actin polymerization or cortical function is impaired. Cyclic-AMP induces transient blebbing independently of much of the known chemotactic signal transduction machinery, but involving PI3-kinase and downstream PH domain proteins, CRAC and PhdA. Impairment of this PI3-kinase pathway results in slow movement under agarose and cells that produce few blebs, though actin polymerization appears unaffected. We propose that mechanical resistance induces bleb-driven movement in Dictyostelium, which is chemotactic and controlled through PI3-kinase.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QK Botany Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Computer Science Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre |
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Library of Congress Subject Headings (LCSH): | Dictyostelium, Chemotaxis, Cell membranes, Blisters | ||||||
Journal or Publication Title: | The Journal of Cell Biology | ||||||
Publisher: | Rockefeller University Press | ||||||
ISSN: | 0021-9525 | ||||||
Official Date: | 10 March 2014 | ||||||
Dates: |
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Volume: | 204 | ||||||
Number: | 6 | ||||||
Page Range: | pp. 1027-1044 | ||||||
DOI: | 10.1083/jcb.201306147 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Date of first compliant deposit: | 12 October 2016 | ||||||
Date of first compliant Open Access: | 12 October 2016 | ||||||
Funder: | Medical Research Council (Great Britain) (MRC), Herchel Smith Fellowship, University of Warwick. Systems Biology Doctoral Training Centre | ||||||
Grant number: | U105115237 (MRC) | ||||||
Embodied As: | 1 |
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