The Library
The role of the environment in transmission of Dichelobacter nodosus between ewes and their lambs
Tools
Muzafar, Mohd, Calvo-Bado, Leo A., Green, Laura E., Smith, Edward M., Russell, Claire L., Grogono-Thomas, Rosemary and Wellington, E. M. H. (2015) The role of the environment in transmission of Dichelobacter nodosus between ewes and their lambs. Veterinary Microbiology, 179 (1-2). pp. 53-59. doi:10.1016/j.vetmic.2015.04.010 ISSN 0378-1135.
|
PDF (Creative Commons : Attribution 4.0)
WRAP_1-s2.0-S0378113515001558-main.pdf - Published Version - Requires a PDF viewer. Download (837Kb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.vetmic.2015.04.010
Abstract
Dichelobacter nodosus (D. nodosus) is the essential causative agent of footrot in sheep. The current study investigated when D. nodosus was detectable on newborn lambs and possible routes of transmission. Specific qPCR was used to detect and quantify the load of D. nodosus in foot swabs of lambs at birth and 5–13 h post-partum, and their mothers 5–13 h post-partum; and in samples of bedding, pasture, soil and faeces. D. nodosus was not detected on the feet of newborn lambs swabbed at birth, but was detected 5–13 h after birth, once they had stood on bedding containing naturally occurring D. nodosus. Multiple genotypes identified by cloning and sequencing a marker gene, pgrA, and by multi locus variable number tandem repeat analysis (MLVA) of community DNA from swabs on individual feet indicated a mixed population of D. nodosus was present on the feet of both ewes and lambs. There was high variation in pgrA tandem repeat number (between 3 and 21 repeats), and multiple MLVA types. The overall similarity index between the populations on ewes and lambs was 0.45, indicating moderate overlap. Mother offspring pairs shared some alleles but not all, suggesting lambs were infected from sources(s) other than just their mother's feet. We hypothesise that D. nodosus is transferred to the feet of lambs via bedding containing naturally occurring populations of D. nodosus, probably as a result of transfer from the feet of the group of housed ewes. The results support the hypothesis that the environment plays a key role in the transmission of D. nodosus between ewes and lambs.
Item Type: | Journal Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Subjects: | S Agriculture > SF Animal culture | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Sheep -- Diseases, Foot -- Wounds and injuries, Footrot in sheep | ||||||||
Journal or Publication Title: | Veterinary Microbiology | ||||||||
Publisher: | Elsevier | ||||||||
ISSN: | 0378-1135 | ||||||||
Official Date: | 31 August 2015 | ||||||||
Dates: |
|
||||||||
Volume: | 179 | ||||||||
Number: | 1-2 | ||||||||
Number of Pages: | 7 | ||||||||
Page Range: | pp. 53-59 | ||||||||
DOI: | 10.1016/j.vetmic.2015.04.010 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 29 December 2015 | ||||||||
Date of first compliant Open Access: | 29 December 2015 | ||||||||
Funder: | Biotechnology and Biological Sciences Research Council (BBSRC), University of Warwick Vice Chancellor Scholarship | ||||||||
Grant number: | BBE01870X1 (BBSRC), 1140257 (UoWVCS) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year