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A rapid and accurate assay for assessing the cytotoxicity of viral proteins
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UNSPECIFIED. (2005) A rapid and accurate assay for assessing the cytotoxicity of viral proteins. JOURNAL OF VIROLOGICAL METHODS, 127 (2). pp. 119-125. ISSN 0166-0934
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Official URL: http://dx.doi.org/10.1016/j.jviromet.2005.03.016
Abstract
A fluorescence-based assay is presented for measuring the cytoxicity of viral proteins added exogenously to cells. The assay is based on the use of two fluorescent dyes, calcein-AM and ethidium homodimer (EtD-1) to specifically stain living and dead cells respectively and employs fluorescence activated cells sorting (FACS) to achieve a rapid and accurate measurement of the cytotoxic capacity of a potential viral toxin. The assay has been developed using the group B homologue (ADRV-NSP4) of the NSP4 enterotoxin encoded by Group A rotaviruses but should be applicable to assaying any viral protein exhibiting cytotoxic activity. (c) 2005 Elsevier B.V. All rights reserved.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology |
| Journal or Publication Title: | JOURNAL OF VIROLOGICAL METHODS |
| Publisher: | ELSEVIER SCIENCE BV |
| ISSN: | 0166-0934 |
| Date: | August 2005 |
| Volume: | 127 |
| Number: | 2 |
| Number of Pages: | 7 |
| Page Range: | pp. 119-125 |
| Identification Number: | 10.1016/j.jviromet.2005.03.016 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/6834 |
Data sourced from Thomson Reuters' Web of Knowledge
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