The Library
A rapid and accurate assay for assessing the cytotoxicity of viral proteins
Tools
UNSPECIFIED (2005) A rapid and accurate assay for assessing the cytotoxicity of viral proteins. JOURNAL OF VIROLOGICAL METHODS, 127 (2). pp. 119-125. doi:10.1016/j.jviromet.2005.03.016 ISSN 0166-0934.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1016/j.jviromet.2005.03.016
Abstract
A fluorescence-based assay is presented for measuring the cytoxicity of viral proteins added exogenously to cells. The assay is based on the use of two fluorescent dyes, calcein-AM and ethidium homodimer (EtD-1) to specifically stain living and dead cells respectively and employs fluorescence activated cells sorting (FACS) to achieve a rapid and accurate measurement of the cytotoxic capacity of a potential viral toxin. The assay has been developed using the group B homologue (ADRV-NSP4) of the NSP4 enterotoxin encoded by Group A rotaviruses but should be applicable to assaying any viral protein exhibiting cytotoxic activity. (c) 2005 Elsevier B.V. All rights reserved.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology |
||||
Journal or Publication Title: | JOURNAL OF VIROLOGICAL METHODS | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0166-0934 | ||||
Official Date: | August 2005 | ||||
Dates: |
|
||||
Volume: | 127 | ||||
Number: | 2 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 119-125 | ||||
DOI: | 10.1016/j.jviromet.2005.03.016 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |