A rapid and accurate assay for assessing the cytotoxicity of viral proteins
UNSPECIFIED. (2005) A rapid and accurate assay for assessing the cytotoxicity of viral proteins. JOURNAL OF VIROLOGICAL METHODS, 127 (2). pp. 119-125. ISSN 0166-0934Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.jviromet.2005.03.016
A fluorescence-based assay is presented for measuring the cytoxicity of viral proteins added exogenously to cells. The assay is based on the use of two fluorescent dyes, calcein-AM and ethidium homodimer (EtD-1) to specifically stain living and dead cells respectively and employs fluorescence activated cells sorting (FACS) to achieve a rapid and accurate measurement of the cytotoxic capacity of a potential viral toxin. The assay has been developed using the group B homologue (ADRV-NSP4) of the NSP4 enterotoxin encoded by Group A rotaviruses but should be applicable to assaying any viral protein exhibiting cytotoxic activity. (c) 2005 Elsevier B.V. All rights reserved.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry
T Technology > TP Chemical technology
Q Science > QR Microbiology > QR355 Virology
|Journal or Publication Title:||JOURNAL OF VIROLOGICAL METHODS|
|Publisher:||ELSEVIER SCIENCE BV|
|Official Date:||August 2005|
|Number of Pages:||7|
|Page Range:||pp. 119-125|
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