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Detection of molecular processes in the intact retina by ATR-FTIR spectromicroscopy
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Massaro, Sebastiano, Zlateva, Theodora, Torre, Vincent and Quaroni, Luca (2007) Detection of molecular processes in the intact retina by ATR-FTIR spectromicroscopy. Analytical and Bioanalytical Chemistry, Volume 390 (Number 1). pp. 317-322. doi:10.1007/s00216-007-1710-4 ISSN 1618-2642.
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Official URL: http://dx.doi.org/10.1007/s00216-007-1710-4
Abstract
We used Fourier transform infrared spectromicroscopy in the attenuated total reflection configuration to study biochemical events associated with the response to light of an intact retina. We show that the technique is suitable for the detection in real time of molecular processes occurring in rod outer segments induced by light absorption. Two-dimensional correlation analysis was applied to the identification and interpretation of specific spectral changes associated to the evolution of the system. The technique allows us to observe an extensive protein translocation, which we interpret as arising from the release of transducin from the disk membrane and its redistribution from the outer segment towards the inner segment of rod cells. These results are in full agreement with our current understanding of retinal physiology and validate the technique as a useful tool for the study of complex molecular processes in intact tissue.
| Item Type: | Journal Article | ||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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| Divisions: | Faculty of Social Sciences > Warwick Business School > Behavioural Science Faculty of Social Sciences > Warwick Business School |
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| Library of Congress Subject Headings (LCSH): | Biochemistry, Physiology, Retina, Infrared equipment | ||||||||||
| Journal or Publication Title: | Analytical and Bioanalytical Chemistry | ||||||||||
| Publisher: | Springer | ||||||||||
| ISSN: | 1618-2642 | ||||||||||
| Official Date: | 7 November 2007 | ||||||||||
| Dates: |
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| Volume: | Volume 390 | ||||||||||
| Number: | Number 1 | ||||||||||
| Number of Pages: | 5 | ||||||||||
| Page Range: | pp. 317-322 | ||||||||||
| DOI: | 10.1007/s00216-007-1710-4 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access |
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