UNSPECIFIED. (2005) A cystic fibrosis epidemic strain of Pseudomonas aeruginosa displays enhanced virulence and antimicrobial resistance. JOURNAL OF BACTERIOLOGY, 187 (14). pp. 4908-4920. ISSN 0021-9193

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Abstract

The Liverpool epidemic strain (LES) of Pseudomonas aeruginosa is a transmissible aggressive pathogen of cystic fibrosis (CF) patients. We compared transcriptome profiles of two LES isolates with each other and with a laboratory and genetic reference strain (PAO1) after growth to late exponential phase and following exposure to oxidative stress. Both LES isolates exhibited enhanced antimicrobial resistances linked to specific mutations in efflux pump genes. Although transcription of AmpC P-lactamase was up-regulated in both, one LES isolate contained a specific mutation rendering the ampC gene untranstatable. The virulence-related quorum-sensing (QS) regulon of LES431, an isolate that caused pneumonia in the non-CF parent of a CF patient, was considerably up-regulated in comparison to either isolate LES400, associated with a chronic CF infection, or strain PAO1. Premature activation of QS genes was detected in isolates from both non-CF parents and the CF patient in a previously reported infection episode. LES isolates lacking the up-regulated QS phenotype contained different frameshift mutations in lasR. When fed to Drosophild melanogaster, isolate LES431 killed the fruit flies more readily than either isolate LES400 or strain PAO1, indicating that virulence varies intraclonally. The LES may represent a clone with enhanced virulence and antimicrobial resistance characteristics that can vary or are lost due to mutations during long-term colonization but have contributed to the successful spread of the lineage throughout the CF population of the United Kingdom.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	JOURNAL OF BACTERIOLOGY
Publisher:	AMER SOC MICROBIOLOGY
ISSN:	0021-9193
Date:	July 2005
Volume:	187
Number:	14
Number of Pages:	13
Page Range:	pp. 4908-4920
Identification Number:	10.1128/JB.187.14.4908-4920.2005
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/6841

Data sourced from Thomson Reuters' Web of Knowledge

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