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Polymerase chain reaction on a viral nanoparticle
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Carr-Smith, James, Pacheco-Gómez, Raúl, Little, Haydn A., Hicks, Matthew R., Sandhu, Sandeep, Steinke, Nadja, Smith, David J., Rodger, Alison, Goodchild, Sarah A., Lukaszewski, Roman A., Tucker, James H. R. and Dafforn, Tim (2015) Polymerase chain reaction on a viral nanoparticle. ACS Synthetic Biology, 4 (12). pp. 1316-1325. doi:10.1021/acssynbio.5b00034 ISSN 2161-5063.
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WRAP_9471544-ch-130615-2015._185._dafforn_viral_particle_pcr_acssynbio2e5b00034.pdf - Accepted Version - Requires a PDF viewer. Download (3427Kb) | Preview |
Official URL: http://dx.doi.org/10.1021/acssynbio.5b00034
Abstract
The field of synthetic biology includes studies that aim to develop new materials and devices from biomolecules. In recent years much work has been carried out using a range of biomolecular chassis including α-helical coiled coils, α-sheet amyloids and even viral particles. In this work we show how hybrid bionanoparticles can be produced from a viral M13 bacteriophage scaffold through conjugation to DNA primers that can template a polymerase chain reaction (PCR). This unprecedented example of a PCR on a virus particle has been studied by flow aligned linear dichroism spectroscopy, which gives information on the structure of the product as well as a new protototype methodology for DNA detection. We propose that this demonstration of PCR on the surface of a bionanoparticle is a useful addition to ways in which hybrid assemblies may be constructed using synthetic biology.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QD Chemistry | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||
Library of Congress Subject Headings (LCSH): | Linear dichromism, Bacteriophages, Nanoparticles | ||||||
Journal or Publication Title: | ACS Synthetic Biology | ||||||
Publisher: | American Chemical Society | ||||||
ISSN: | 2161-5063 | ||||||
Official Date: | 5 June 2015 | ||||||
Dates: |
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Volume: | 4 | ||||||
Number: | 12 | ||||||
Page Range: | pp. 1316-1325 | ||||||
DOI: | 10.1021/acssynbio.5b00034 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Date of first compliant deposit: | 13 January 2016 | ||||||
Date of first compliant Open Access: | 27 June 2016 | ||||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||
Grant number: | EP/G007578/1 (EPSRC), BB/J02001X/1 (BBSRC), EP/I502025/1 (EPSRC), EP/G005869/1 (EPSRC) |
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