Lobo, Daniela P., Wemyss, Alan M., Smith, David J., Straube, Anne, Betteridge, Kai B., Salmon, Andrew H. J., Foster, Rebecca R., Elhegni, Hesham E., Satchell, Simon C., Little, Haydn A., Pacheco-Gómez, Raúl, Simmons, Mark J., Hicks, Matthew R., Bates, David O., Rodger, Alison, Dafforn, Tim and Arkill, Kenton P. (2015) Direct detection and measurement of wall shear stress using a filamentous bio-nanoparticle. Nano Research, 8 (10). pp. 3307-3315. doi:10.1007/s12274-015-0831-x ISSN 1998-0124.

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Abstract

The wall shear stress (WSS) that a moving fluid exerts on a surface affects many processes including those relating to vascular function. WSS plays an important role in normal physiology (e.g. angiogenesis) and affects the microvasculature's primary function of molecular transport. It is known that points of fluctuating WSS show abnormalities in a number of diseases, however, there is no established technique for measuring WSS directly in physiological systems. All current methods rely on estimates obtained from measured velocity gradients in bulk flow data. In this work we report a nanosensor that can directly measure WSS in microfluidic chambers with sub-micron spatial resolution using a specific type of virus, the bacteriophage M13, which has been fluorescently labelled and anchored to a surface. It is demonstrated that the nanosensor can be calibrated and adapted for biological tissue, giving results that show WSS in micro-domains of cells that cannot be calculated accurately from bulk flow measurements. This method lends itself to a platform applicable to many applications in biology and microfluidics.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Research Centres > Molecular Organisation and Assembly in Cells (MOAC) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Bacteriophages, Nanoparticles
Journal or Publication Title:	Nano Research
Publisher:	Tsinghua University Press
ISSN:	1998-0124
Official Date:	October 2015
Dates:	Date Event October 2015 Published 25 August 2015 Available 8 June 2015 Accepted
Volume:	8
Number:	10
Number of Pages:	14
Page Range:	pp. 3307-3315
DOI:	10.1007/s12274-015-0831-x
Status:	Peer Reviewed
Publication Status:	Published
Description:	Electronic supplementary material
Date of first compliant deposit:	13 January 2016

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