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Direct detection and measurement of wall shear stress using a filamentous bio-nanoparticle
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Lobo, Daniela P., Wemyss, Alan M., Smith, David J., Straube, Anne, Betteridge, Kai B., Salmon, Andrew H. J., Foster, Rebecca R., Elhegni, Hesham E., Satchell, Simon C., Little, Haydn A., Pacheco-Gómez, Raúl, Simmons, Mark J., Hicks, Matthew R., Bates, David O., Rodger, Alison, Dafforn, Tim and Arkill, Kenton P. (2015) Direct detection and measurement of wall shear stress using a filamentous bio-nanoparticle. Nano Research, 8 (10). pp. 3307-3315. doi:10.1007/s12274-015-0831-x ISSN 1998-0124.
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WRAP_9471544-ch-130615-1506_wss_nano_res_template_.pdf Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (7Mb) |
Official URL: http://dx.doi.org/10.1007/s12274-015-0831-x
Abstract
The wall shear stress (WSS) that a moving fluid exerts on a surface affects many processes including those relating to vascular function. WSS plays an important role in normal physiology (e.g. angiogenesis) and affects the microvasculature's primary function of molecular transport. It is known that points of fluctuating WSS show abnormalities in a number of diseases, however, there is no established technique for measuring WSS directly in physiological systems. All current methods rely on estimates obtained from measured velocity gradients in bulk flow data. In this work we report a nanosensor that can directly measure WSS in microfluidic chambers with sub-micron spatial resolution using a specific type of virus, the bacteriophage M13, which has been fluorescently labelled and anchored to a surface. It is demonstrated that the nanosensor can be calibrated and adapted for biological tissue, giving results that show WSS in micro-domains of cells that cannot be calculated accurately from bulk flow measurements. This method lends itself to a platform applicable to many applications in biology and microfluidics.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QD Chemistry | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Research Centres > Molecular Organisation and Assembly in Cells (MOAC) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Bacteriophages, Nanoparticles | ||||||||
Journal or Publication Title: | Nano Research | ||||||||
Publisher: | Tsinghua University Press | ||||||||
ISSN: | 1998-0124 | ||||||||
Official Date: | October 2015 | ||||||||
Dates: |
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Volume: | 8 | ||||||||
Number: | 10 | ||||||||
Number of Pages: | 14 | ||||||||
Page Range: | pp. 3307-3315 | ||||||||
DOI: | 10.1007/s12274-015-0831-x | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Description: | Electronic supplementary material |
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Date of first compliant deposit: | 13 January 2016 |
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