Millard, Andrew D. and Mann, Nicholas H.. (2006) A temporal and spatial investigation of cyanophage abundance in the Gulf of Aqaba Red Sea. Journal of the Marine Biological Association of the United Kingdom, Vol.86 (No.3). pp. 507-515. ISSN 0025-3154

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Abstract

The aim of this study was to determine the abundance of cyanophages over an annual cycle in the Red Sea from the period April 1999 to December 1999 at a range of depths. Cyanophage numbers from 71 water samples were determined by the use of plaque assays using four different Synechococcus strains. The results indicate that cyanophage are found throughout the water column from surface waters to depths of 150 m, with a discrete maximum in the number of cyanophages in the summer months of July, August and September at a depth of 30 m. Eighty-seven cyanophages were isolated and characterized in terms of host range, genome size and possession of a myoviral portal vertex gene. Cyanophages were found to infect multiple strains of Synechococcus from different phylogenetic clades. The genome sizes of cyanophages were also found to be bigger than previously estimated.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Cyanobacteria -- Aqaba, Gulf of, Bacteriophages -- Research, Bacteriophages -- Genetics, Ecology -- Aqaba, Gulf of
Journal or Publication Title:	Journal of the Marine Biological Association of the United Kingdom
Publisher:	Cambridge University Press
ISSN:	0025-3154
Date:	June 2006
Volume:	Vol.86
Number:	No.3
Page Range:	pp. 507-515
Identification Number:	10.1017/S0025315406013415
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Natural Environment Research Council (Great Britain) (NERC)
References:	Delbruck, M., 1940. The growth of bacteriophage and lysis of the host. Journal of General Physiology, 23, 643-660. Frederickson, C.M., Short, S.M. & Suttle, C.A., 2003. The physical environment affects cyanophage communities in British Columbia inlets. Microbial Ecology, 46, 348-357. Fuller, N.J., Marie, D., Partensky, F., Vaulot, D., Post, A.F. & Scanlan, D.J., 2003. Clade-specific 16S ribosomal DNA oligonucleotides reveal the predominance of a single marine Synechococcus clade throughout a stratified water column in the Red Sea. Applied and Environmental Microbiology, 69, 2430-2443. Fuller, N.J., West, N.J., Marie, D., Yallop, M., Rivlin, T., Post, A.F. & Scanlan, D.J., 2005. Dynamics of community structure and phosphate status of picocyanobacterial populations in the Gulf of Aqaba, Red Sea. Limnology and Oceanography, 50, 363-375. Fuller, N.J., Wilson, W.H., Joint, I.R. & Mann, N.H., 1998. Occurrence of a sequence in marine cyanophages similar to that of T4 g20 and its application to PCR-based detection and quanti¢cation techniques. Applied and Environmental Microbiology, 64, 2051-2060. Hambly, E.,Te¤tart, F., Desplats, C.,Wilson,W.H., Krisch, H.M. & Mann, N.H., 2001. A conserved genetic module that encodes the major virion components in both the coliphage T4 and the marine cyanophage S-PM2. Proceedings of the National Academy of Sciences of the United States of America, 98, 11411-11416. Herdman, M., Castenholz, R.W., Iteman, I.,Waterbury, J.B. & Rippka, R., 2001. The Archaea and the deeply branching and phototrophic bacteria. In Bergey’s manual of systematic biology (ed. D.R. Boone et al.), pp. 493-514. Springer Publishers. Klinker, J., Reiss, Z., Kropach, C., Levanon, I., Harpaz, H. & Halicz, E., 1976. Observations on the circulation pattern in the Gulf of Elat (Aqaba), Red Sea. Israel Journal of Earth Sciences, 25, 85-103. Lu, J., Chen, F. & Hodson, R.E., 2001. Distribution, isolation, host specificity, and diversity of cyanophages infecting marine Synechococcus spp. in river estuaries. Applied and Environmental Microbiology, 67, 3285-3290. Mann, N.H., Clokie,M.R., Millard, A., Cook, A.,Wilson,W.H., Wheatley, P.J., Letarov, A. & Krisch, H.M., 2005. The genome of S-PM2, a ‘photosynthetic’ T4-type bacteriophage that infects marine Synechococcus strains. Journal of Bacteriology, 187, 3188-3200. Mann, N.H., Cook, A., Millard, A., Bailey, S. & Clokie, M., 2003. Marine ecosystems: bacterial photosynthesis genes in a virus. Nature, London, 424, 741-741. Marston, M.F. & Sallee, J.L., 2003. Genetic diversity and temporal variation in the cyanophage community infecting marine Synechococcus species in Rhode Island’s coastal waters. Applied and Environmental Microbiology, 69, 4639-4647. Müling, M. et al., 2005. Genetic diversity of marine Synechococcus and co-occurring cyanophage communities: evidence for viral control of phytoplankton. Environmental Microbiology, 7, 499-508. Partensky, F., Hess, W.R. & Vaulot, D., 1999. Prochlorococcus, a marine photosynthetic prokaryote of global signi¢cance. Microbiology and Molecular Biology Reviews, 63, 106-127. Reiss, Z. & Hottinger, L., 1984.The Gulf of Aqaba (Elat): ecological micropaleontology (Ecological Studies,Vol. 50). NewYork: Springer-Verlag. Sullivan, M.B., Waterbury, J.B. & Chisholm, S.W., 2003. Cyanophages infecting the oceanic cyanobacterium Prochlorococcus. Nature, London, 424, 1047^1051. Suttle, C.A., 2000. Cyanophages and their role in the ecology of cyanobacteria. In the ecology of cyanobacteria: their diversity in time and space (ed. B.A. Whitton and M. Potts). Boston: Kluwer Academic Publishers. Suttle, C.A. & Chan, A.M., 1993. Marine cyanophages infecting oceanic and coastal strains of Synechococcus-abundance, morphology, cross-infectivity and growth characteristics. Marine Ecology Progress Series, 92, 99-109. Suttle, C.A. & Chan, A.M., 1994. Dynamics and distribution of cyanophages and their e¡ect on marine Synechococcus spp. Applied and Environmental Microbiology, 60, 3167-3174. Waterbury, J. & Rippka, R., 1989. The order Chroococcales. In Bergey’s Manual of Systematic Bacteriology (ed. J.T. Staley et al.), pp. 1728-1746. Baltimore, Williams & Wilkins. Waterbury, J.B. & Valois, F.W., 1993. Resistance to cooccurring phages enables marine Synechococcus communities to coexist with cyanophages abundant in seawater. Applied and Environmental Microbiology, 59, 3393-3399. Wilson, W.H., Joint, I.R., Carr, N.G. & Mann, N.H., 1993. Isolation and molecular characterization of 5 marine cyanophages propagated on Synechococcus sp strainWH7803. Applied and Environmental Microbiology, 59, 3736-3743. Wolfvecht, A., Paldor, N. & Brenner, S., 1992. Hydrographic indications of advection convection effects in the Gulf of Elat. Deep-Sea Research Part A-Oceanographic Research Papers, 39, 1393-1401. Wommack, K.E. & Colwell, R.R., 2000.Virioplankton: viruses in aquatic ecosystems. Microbiology and Molecular Biology Reviews, 64, 69-114. Wyman, M., Gregory, R.P.F. & Carr, N.G., 1985. Novel role of phycoerythrin in a marine cyanobacterium, Synechococcus strain DC2. Science, New York, 230, 818-820.
URI:	http://wrap.warwick.ac.uk/id/eprint/689

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