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The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle
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Nixon, Faye M., Gutierrez-Caballero, Cristina, Hood, Fiona E., Booth, Daniel G., Prior, Ian A. and Royle, Stephen J. (2015) The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. eLife, 4 . pp. 1-21. 07635. doi:10.7554/eLife.07635 ISSN 2050-084X.
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Official URL: http://dx.doi.org/10.7554/eLife.07635
Abstract
Kinetochore fibers (K-fibers) of the mitotic spindle are force-generating units that power chromosome movement during mitosis. K-fibers are composed of many microtubules that are held together throughout their length. Here we show, using 3D electron microscopy, that K-fiber microtubules are connected by a network of microtubule connectors. We term this network 'the mesh'. The K-fiber mesh is made of linked multipolar connectors. Each connector has up to four struts, so that a single connector can link up to four microtubules. Molecular manipulation of the mesh by overexpression of TACC3 causes disorganization of the K-fiber microtubules. Optimal stabilization of K-fibers by the mesh is required for normal progression through mitosis. We propose that the mesh stabilizes K-fibers by pulling MTs together and thereby maintaining the integrity of the fiber. Our work thus identifies the K-fiber meshwork of linked multipolar connectors as a key integrator and determinant of K-fiber structure and function.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Cell cycle, Ultrastructure (Biology) | ||||||||
Journal or Publication Title: | eLife | ||||||||
Publisher: | eLife Sciences Publications Ltd. | ||||||||
ISSN: | 2050-084X | ||||||||
Official Date: | 19 June 2015 | ||||||||
Dates: |
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Volume: | 4 | ||||||||
Number of Pages: | 21 | ||||||||
Page Range: | pp. 1-21 | ||||||||
Article Number: | 07635 | ||||||||
DOI: | 10.7554/eLife.07635 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 29 December 2015 | ||||||||
Date of first compliant Open Access: | 29 December 2015 | ||||||||
Funder: | North West Cancer Research, Cancer Research UK (CRUK) | ||||||||
Grant number: | CR928 (NWCR), C25425/A15182 (CRUK) |
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