Fisher, Ffolliott M., Trujillo, M. E., Hanif, W., Barnett, A. H., McTernan, P. G. (Philip G.), Scherer, P. E. and Kumar, Sudhesh. (2005) Serum high molecular weight complex of adiponectin correlates better with glucose tolerance than total serum adiponectin in Indo-Asian males. Diabetologia, Volume 48 (Number 6). pp. 1084-1087. ISSN 0012-186X

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Abstract

It is well established that total systemic adiponectin is reduced in type 2 diabetic subjects. To date most studies have been concerned with the singular full-length protein or proteolytically cleaved globular domain. It is, however, apparent that the native protein circulates in serum as a lower molecular weight hexamer and as larger multimeric structures of high molecular weight (HMW). In this study we address the clinical significance of each form of the protein with respect to glucose tolerance.Serum was obtained from 34 Indo-Asian male subjects (BMI 26.5 +/- 3.1; age 52.15 +/- 10.14 years) who had undertaken a 2-h oral glucose tolerance test. An aliquot of serum was fractionated using velocity sedimentation followed by reducing SDS-PAGE. Western blots were probed for adiponectin, and HMW adiponectin as a percentage of total adiponectin (percentage of higher molecular weight adiponectin [S-A] index) was calculated from densitometry readings. Total adiponectin was measured using ELISA; leptin, insulin and IL-6 were determined using ELISA. Analysis of the cohort demonstrated that total adiponectin (r=0.625, p=0.0001), fasting insulin (r=0.354, p=0.040) and age (r=0.567, p=0.0001) correlated with S-A. S-A showed a tighter, inverse correlation with 2-h glucose levels (r=0.58, p=0.0003) than total adiponectin (r=0.38, p=0.0001). This study demonstrates the importance of the S-A index as a better determinant of glucose intolerance than measurements of total adiponectin. Our findings suggest that HMW adiponectin is the active form of the protein.

Item Type:	Journal Article
Subjects:	R Medicine > RC Internal medicine
Divisions:	Faculty of Medicine > Warwick Medical School > Translational & Systems Medicine > Metabolic and Vascular Health Faculty of Medicine > Warwick Medical School
Journal or Publication Title:	Diabetologia
Publisher:	Springer
ISSN:	0012-186X
Official Date:	June 2005
Volume:	Volume 48
Number:	Number 6
Number of Pages:	4
Page Range:	pp. 1084-1087
Identification Number:	10.1007/s00125-005-1758-7
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/6932

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