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Molecular basis of sugar recognition by collectin-K1 and the effects of mutations associated with 3MC syndrome
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Girija, Umakhanth Venkatraman, Furze, Christopher M., Gingras, Alexandre R., Yoshizak, Takayuki, Ohanti, Katsuki, Marshall, Jamie E., Wallis, A. Katrine, Schwaeble, Wilhelm J., El-Mezgaeldi, Mohammed, Mitchell, Daniel A., Moody, Peter C. E., Wakamiya, Nobutaka and Wallis, Russell (2015) Molecular basis of sugar recognition by collectin-K1 and the effects of mutations associated with 3MC syndrome. Biomed Central Public Health, Volume 13 . pp. 1-13. 27. doi:10.1186/s12915-015-0136-2 ISSN 1471-2458.
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Official URL: http://dx.doi.org/10.1186/s12915-015-0136-2
Abstract
Background:
Collectin-K1 (CL-K1, or CL-11) is a multifunctional Ca2+-dependent lectin with roles in innate immunity, apoptosis and embryogenesis. It binds to carbohydrates on pathogens to activate the lectin pathway of complement and together with its associated serine protease MASP-3 serves as a guidance cue for neural crest development. High serum levels are associated with disseminated intravascular coagulation, where spontaneous clotting can lead to multiple organ failure. Autosomal mutations in the CL-K1 or MASP-3 genes cause a developmental disorder called 3MC (Carnevale, Mingarelli, Malpuech and Michels) syndrome, characterised by facial, genital, renal and limb abnormalities. One of these mutations (Gly204Ser in the CL-K1 gene) is associated with undetectable levels of protein in the serum of affected individuals.
Results:
In this study, we show that CL-K1 primarily targets a subset of high-mannose oligosaccharides present on both self- and non-self structures, and provide the structural basis for its ligand specificity. We also demonstrate that three disease-associated mutations prevent secretion of CL-K1 from mammalian cells, accounting for the protein deficiency observed in patients. Interestingly, none of the mutations prevent folding or oligomerization of recombinant fragments containing the mutations in vitro. Instead, they prevent Ca2+ binding by the carbohydrate-recognition domains of CL-K1. We propose that failure to bind Ca2+ during biosynthesis leads to structural defects that prevent secretion of CL-K1, thus providing a molecular explanation of the genetic disorder.
Conclusions:
We have established the sugar specificity of CL-K1 and demonstrated that it targets high-mannose oligosaccharides on self- and non-self structures via an extended binding site which recognises the terminal two mannose residues of the carbohydrate ligand. We have also shown that mutations associated with a rare developmental disorder called 3MC syndrome prevent the secretion of CL-K1, probably as a result of structural defects caused by disruption of Ca2+ binding during biosynthesis.
| Item Type: | Journal Article | ||||||||
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| Subjects: | R Medicine > RC Internal medicine | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Health Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Lectins | ||||||||
| Journal or Publication Title: | Biomed Central Public Health | ||||||||
| Publisher: | BioMed Central Ltd | ||||||||
| ISSN: | 1471-2458 | ||||||||
| Official Date: | 17 April 2015 | ||||||||
| Dates: |
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| Volume: | Volume 13 | ||||||||
| Number of Pages: | 13 | ||||||||
| Page Range: | pp. 1-13 | ||||||||
| Article Number: | 27 | ||||||||
| DOI: | 10.1186/s12915-015-0136-2 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 30 December 2015 | ||||||||
| Date of first compliant Open Access: | 30 December 2015 | ||||||||
| Funder: | Medical Research Council (Great Britain) (MRC), American Heart Association (AHA), Nihon Gakujutsu Shinkōkai [Japan Society for the Promotion of Science] (NGS), National Institute of General Medical Sciences (U.S.) (NIGMS) | ||||||||
| Grant number: | G1000191/1 (MRC), 12SDG11610043 (AHA), 26293124 (NGS), GM62116 (NIGMS) |
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