UNSPECIFIED. (2005) The detection and characterisation of Dichelobacter nodosus from cases of ovine footrot in England and Wales. VETERINARY MICROBIOLOGY, 108 (1-2). pp. 57-67. ISSN 0378-1135

Full text not available from this repository.

Abstract

Footrot, caused by the strictly anaerobic bacterium Dichelobacter nodosus, is the most common cause of lameness in sheep in Great Britain but problems exist in association with its diagnosis and control. The fastidious nature of D. nodosus means that complex media and several weeks are required for characterisation. An alternative method to simplify and enhance the detection of D. nodosus in clinical samples is therefore highly desirable. In terms of control, anecdotal evidence from the farming community suggests that the commercially available vaccine, based on Australian isolates of D. nodosus, is not widely employed in this country due to its perceived inefficacy. Seven hundred and six isolates, collected from outbreaks in England and Wales, were therefore used to investigate these issues. A 16S rRNA PCR was adapted to detect D. nodosus in clinical material within 1 day of sampling; a 15% increase in detection compared with culture and less than 1% false negatives were achieved. This represents a major advance in the rapid diagnosis of footrot and will be of great value to practitioners and diagnostic laboratories. Bacterial virulence was tested using protease thermostability and zymogram assays, whilst serogrouping was performed by slide agglutination. All isolates demonstrated virulence patterns previously recorded in Australia and all nine serogroups of D. nodosus (A-I) were represented. Serogroup H was predominant. There was, therefore, no evidence for the presence of novel strains of D. nodosus compared with Australia suggesting the need for further investigation into farmers' views on the use of the commercial vaccine in Great Britain. (c) 2005 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology S Agriculture > SF Animal culture
Journal or Publication Title:	VETERINARY MICROBIOLOGY
Publisher:	ELSEVIER SCIENCE BV
ISSN:	0378-1135
Date:	15 June 2005
Volume:	108
Number:	1-2
Number of Pages:	11
Page Range:	pp. 57-67
Identification Number:	10.1016/j.vetmic.2005.01.029
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/6956

Data sourced from Thomson Reuters' Web of Knowledge

Request changes to a record

Actions (login required)

View Item