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Enzyme characterisation and kinetic modelling of the pentose 2 phosphate pathway in yeast
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Messiha, Hanan L., Kent, Edward, Malys, Naglis, Carroll, Kathleen M., Swainston, Neil, Mendes, Pedro and Smalbone, Kieran (2014) Enzyme characterisation and kinetic modelling of the pentose 2 phosphate pathway in yeast. PeerJ PrePrints, 4 (146). e146v4.
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Official URL: https://dx.doi.org/10.7287/peerj.preprints.146v4
Abstract
We present the quantification and kinetic characterisation of the enzymes of the pentose phosphate pathway in Saccharomyces cerevisiae. The data are combined into a mathematical model that describes the dynamics of this system and allows us to predict changes in metabo- ite concentrations and fluxes in response to perturbations. We use the model to study theresponse of yeast to a glucose pulse. We then combine the model with an existing glycolysis model to study the effect of oxidative stress on carbohydrate metabolism. The combina-tion of these two models was made possible by the standardised enzyme kinetic experiments carried out in both studies. This work demonstrates the feasibility of constructing larger network-scale models by merging smaller pathway-scale models
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Pentose phosphate pathway | ||||
| Journal or Publication Title: | PeerJ PrePrints | ||||
| Publisher: | PeerJ, Ltd. | ||||
| ISSN: | 2167-9843 | ||||
| Official Date: | 10 April 2014 | ||||
| Dates: |
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| Volume: | 4 | ||||
| Number: | 146 | ||||
| Number of Pages: | 30 | ||||
| Article Number: | e146v4 | ||||
| Status: | Not Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Seventh Framework Programme (European Commission) (FP7), National Institutes of Health (U.S.) (NIH) | ||||
| Grant number: | BB/C008219/1 (BBSRC), BB/G529859 (BBSRC), 289434 (FP7), BB/J019259 (BBSRC), BB/K019783 (BBSRC), R01-GM080219 (NIH) |
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