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Enzyme characterisation and kinetic modelling of the pentose 2 phosphate pathway in yeast

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Messiha, Hanan L., Kent, Edward, Malys, Naglis, Carroll, Kathleen M., Swainston, Neil, Mendes, Pedro and Smalbone, Kieran (2014) Enzyme characterisation and kinetic modelling of the pentose 2 phosphate pathway in yeast. PeerJ PrePrints, 4 (146). e146v4.

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Official URL: https://dx.doi.org/10.7287/peerj.preprints.146v4

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Abstract

We present the quantification and kinetic characterisation of the enzymes of the pentose phosphate pathway in Saccharomyces cerevisiae. The data are combined into a mathematical model that describes the dynamics of this system and allows us to predict changes in metabo- ite concentrations and fluxes in response to perturbations. We use the model to study theresponse of yeast to a glucose pulse. We then combine the model with an existing glycolysis model to study the effect of oxidative stress on carbohydrate metabolism. The combina-tion of these two models was made possible by the standardised enzyme kinetic experiments carried out in both studies. This work demonstrates the feasibility of constructing larger network-scale models by merging smaller pathway-scale models

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Pentose phosphate pathway
Journal or Publication Title: PeerJ PrePrints
Publisher: PeerJ, Ltd.
ISSN: 2167-9843
Official Date: 10 April 2014
Dates:
DateEvent
10 April 2014Published
Volume: 4
Number: 146
Number of Pages: 30
Article Number: e146v4
Status: Not Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
Funder: Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Seventh Framework Programme (European Commission) (FP7), National Institutes of Health (U.S.) (NIH)
Grant number: BB/C008219/1 (BBSRC), BB/G529859 (BBSRC), 289434 (FP7), BB/J019259 (BBSRC), BB/K019783 (BBSRC), R01-GM080219 (NIH)

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