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A new frontier in synthetic biology : automated design of small RNA devices in bacteria
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Rodrigo, Guillermo, Landrain, Thomas E., Shen, Shensi and Jaramillo, Alfonso (2013) A new frontier in synthetic biology : automated design of small RNA devices in bacteria. Trends in Genetics, Volume 29 (Number 9). pp. 529-536. doi:10.1016/j.tig.2013.06.005 ISSN 0168-9525.
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Official URL: http://dx.doi.org/10.1016/j.tig.2013.06.005
Abstract
RNA devices provide synthetic biologists with tools for manipulating post-transcriptional regulation and conditional detection of cellular biomolecules. The use of computational methods to design RNA devices has improved to the stage where it is now possible to automate the entire design process. These methods utilize structure prediction tools that optimize nucleotide sequences, together with fragments of known independent functionalities. Recently, this approach has been used to create an automated method for the de novo design of riboregulators. Here, we describe how it is possible to obtain riboregulatory circuits in prokaryotes by capturing the relevant interactions of RNAs inside the cytoplasm using a physicochemical model. We focus on the regulation of protein expression mediated by intra- or intermolecular interactions of small RNAs (sRNAs), and discuss the design of riboregulators for other functions. The automated design of RNA devices opens new possibilities for engineering fully synthetic regulatory systems that program new functions or reprogram dysfunctions in living cells.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QH Natural history | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Library of Congress Subject Headings (LCSH): | Synthetic biology, RNA | ||||||
Journal or Publication Title: | Trends in Genetics | ||||||
Publisher: | Elsevier Ltd. | ||||||
ISSN: | 0168-9525 | ||||||
Official Date: | September 2013 | ||||||
Dates: |
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Volume: | Volume 29 | ||||||
Number: | Number 9 | ||||||
Number of Pages: | 8 | ||||||
Page Range: | pp. 529-536 | ||||||
DOI: | 10.1016/j.tig.2013.06.005 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access |
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