UNSPECIFIED (2005) Fluorescence in situ hybridisation (FISH) - the next generation. FEMS MICROBIOLOGY LETTERS, 246 (2). pp. 151-158. ISSN 0378-1097

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Abstract

Fluorescence in situ hybridisation (FISH) has become one of the major techniques in environmental microbiology. The original version of this technique often suffered from limited sensitivity due to low target copy number or target inaccessibility. In recent years there have been several developments to amend this problem by increasing signal intensity. This review summarises various approaches for signal amplification, focussing especially on two widely recognised varieties, tyramide signal amplification and multiply labelled polynucleotide probes. Furthermore, new applications for FISH are discussed, which arise from the increased sensitivity of the method. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Item Type:	Journal Item
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	FEMS MICROBIOLOGY LETTERS
Publisher:	ELSEVIER SCIENCE BV
ISSN:	0378-1097
Date:	15 May 2005
Volume:	246
Number:	2
Number of Pages:	8
Page Range:	pp. 151-158
Identification Number:	10.1016/j.femsle.2005.04.015
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/6999

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