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Measurement of glyoxalase gene expression
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Xue, Mingzhan, Rabbani, Naila and Thornalley, Paul J. (2014) Measurement of glyoxalase gene expression. Biochemical Society Transactions, 42 (2). pp. 495-499. 495. doi:10.1042/BST20140026 ISSN 0300-5127.
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Official URL: http://dx.doi.org/10.1042/BST20140026
Abstract
The glyoxalase system is an important component of the enzymatic defence against glycation, preventing particularly quantitatively and functionally important glycation of protein and DNA by methylglyoxal. Expression of genes encoding Glo1 (glyoxalase I) and Glo2 (glyoxalase II) may be induced or suppressed, and rates of proteolysis of Glo1 and Glo2 proteins may change in health and disease. Quantitative assessment of glyoxalase gene expression at the mRNA and protein levels has become a key part of glyoxalase system characterization. For mRNA, there is the common technique of real-time RT (reverse transcription)-PCR and direct quantification of mRNA copy number by the Nanostring™ method. For glyoxalase protein quantification, there is the commonly used Western blotting, and also immunoassay and, in proteome-wide studies, quantitative proteomics and proteome dynamics. We provide protocols for the common methods below and briefly review their application.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QR Microbiology | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Health Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Glyoxalase, Gene expression | ||||||
Journal or Publication Title: | Biochemical Society Transactions | ||||||
Publisher: | Portland Press Ltd. | ||||||
ISSN: | 0300-5127 | ||||||
Official Date: | 1 April 2014 | ||||||
Dates: |
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Volume: | 42 | ||||||
Number: | 2 | ||||||
Number of Pages: | 5 | ||||||
Page Range: | pp. 495-499 | ||||||
Article Number: | 495 | ||||||
DOI: | 10.1042/BST20140026 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) |
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