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Stable isotope probing - linking microbial identity to function
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UNSPECIFIED (2005) Stable isotope probing - linking microbial identity to function. Nature, 3 (6). pp. 499-504. ISSN 1740-1526
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Official URL: http://dx.doi.org/10.1038/nrmicro1162
Abstract
Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as C-13, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and C-13-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics.
| Item Type: | Journal Item |
|---|---|
| Subjects: | Q Science > QR Microbiology |
| Journal or Publication Title: | Nature |
| Publisher: | NATURE PUBLISHING GROUP |
| ISSN: | 1740-1526 |
| Date: | June 2005 |
| Volume: | 3 |
| Number: | 6 |
| Number of Pages: | 6 |
| Page Range: | pp. 499-504 |
| Identification Number: | 10.1038/nrmicro1162 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/7010 |
Data sourced from Thomson Reuters' Web of Knowledge
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