Stable isotope probing - linking microbial identity to function
UNSPECIFIED (2005) Stable isotope probing - linking microbial identity to function. Nature, 3 (6). pp. 499-504. ISSN 1740-1526Full text not available from this repository.
Official URL: http://dx.doi.org/10.1038/nrmicro1162
Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as C-13, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and C-13-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics.
|Item Type:||Journal Item|
|Subjects:||Q Science > QR Microbiology|
|Journal or Publication Title:||Nature|
|Publisher:||NATURE PUBLISHING GROUP|
|Number of Pages:||6|
|Page Range:||pp. 499-504|
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