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Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure
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Webber, Mark A., Whitehead, Rebekah N., Mount, Manuella, Loman, Nicholas J., Pallen, Mark J. and Piddock, Laura J. V. (2015) Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure. Journal of antimicrobial chemotherapy, Volume 70 (Number 8). pp. 2241-2248. doi:10.1093/jac/dkv109 ISSN 0305-7453.
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Official URL: http://dx.doi.org/10.1093/jac/dkv109
Abstract
Objectives:
Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance.
Methods:
Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance.
Results:
All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan.
Conclusions:
This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Drug resistance in microorganisms | ||||||||||
Journal or Publication Title: | Journal of antimicrobial chemotherapy | ||||||||||
Publisher: | Oxford University Press | ||||||||||
ISSN: | 0305-7453 | ||||||||||
Official Date: | August 2015 | ||||||||||
Dates: |
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Volume: | Volume 70 | ||||||||||
Number: | Number 8 | ||||||||||
Number of Pages: | 8 | ||||||||||
Page Range: | pp. 2241-2248 | ||||||||||
DOI: | 10.1093/jac/dkv109 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||
Date of first compliant deposit: | 30 December 2015 | ||||||||||
Date of first compliant Open Access: | 30 December 2015 | ||||||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||||||
Grant number: | BB/GO12016/1 | ||||||||||
Adapted As: |
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