UNSPECIFIED. (2005) Molecular characterization of the rotavirus NSP4 enterotoxin homologue from group B rotavirus. VIRUS RESEARCH, 110 (1-2). pp. 151-160. ISSN 0168-1702

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Abstract

The RNA segment (Gene 10) from a human group B rotavirus which encodes the homologue of the rotavirus enterotoxin (NSP4) has been cloned and sequenced. The gene is of the same length (751 nucleotides) as its better-characterized group A rotavirus counterpart but shows minimal homology (similar to 10%) to it at the primary sequence level. Despite this low level of sequence homology, secondary structure predictions for the group B protein (ADRV-NSP4) showed a close similarity of structural features with the group A protein. Full-length ADRV-NSP4 was expressed in Escherichia coli with an amino terminal 6xHis tag that was used to purify it to homogeneity. The cytotoxicity of the purified protein was examined in a rapid dye-uptake assay that assesses membrane permeability and was found to be comparable to its group A counterpart. (c) 2005 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title:	VIRUS RESEARCH
Publisher:	ELSEVIER SCIENCE BV
ISSN:	0168-1702
Date:	June 2005
Volume:	110
Number:	1-2
Number of Pages:	10
Page Range:	pp. 151-160
Identification Number:	10.1016/j.virusres.2005.02.005
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/7032

Data sourced from Thomson Reuters' Web of Knowledge

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