Molecular characterization of the rotavirus NSP4 enterotoxin homologue from group B rotavirus
UNSPECIFIED. (2005) Molecular characterization of the rotavirus NSP4 enterotoxin homologue from group B rotavirus. VIRUS RESEARCH, 110 (1-2). pp. 151-160. ISSN 0168-1702Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.virusres.2005.02.005
The RNA segment (Gene 10) from a human group B rotavirus which encodes the homologue of the rotavirus enterotoxin (NSP4) has been cloned and sequenced. The gene is of the same length (751 nucleotides) as its better-characterized group A rotavirus counterpart but shows minimal homology (similar to 10%) to it at the primary sequence level. Despite this low level of sequence homology, secondary structure predictions for the group B protein (ADRV-NSP4) showed a close similarity of structural features with the group A protein. Full-length ADRV-NSP4 was expressed in Escherichia coli with an amino terminal 6xHis tag that was used to purify it to homogeneity. The cytotoxicity of the purified protein was examined in a rapid dye-uptake assay that assesses membrane permeability and was found to be comparable to its group A counterpart. (c) 2005 Elsevier B.V. All rights reserved.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology > QR355 Virology|
|Journal or Publication Title:||VIRUS RESEARCH|
|Publisher:||ELSEVIER SCIENCE BV|
|Number of Pages:||10|
|Page Range:||pp. 151-160|
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