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Circadian variation of the human metabolome captured by real-time breath analysis
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Martinez-Lozano Sinues, Pablo, Tarokh, Leila, Li, Xue, Kohler, Malcolm, Brown, Steven A., Zenobi, Renato and Dallmann, Robert (2014) Circadian variation of the human metabolome captured by real-time breath analysis. PLoS One, 9 (12). pp. 1-16. e114422. doi:10.1371/journal.pone.0114422
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Official URL: http://dx.doi.org/10.1371/journal.pone.0114422
Abstract
Circadian clocks play a significant role in the correct timing of physiological metabolism, and clock disruption might lead to pathological changes of metabolism. One interesting method to assess the current state of metabolism is metabolomics. Metabolomics tries to capture the entirety of small molecules, i.e. the building blocks of metabolism, in a given matrix, such as blood, saliva or urine. Using mass spectrometric approaches we and others have shown that a significant portion of the human metabolome in saliva and blood exhibits circadian modulation; independent of food intake or sleep/wake rhythms. Recent advances in mass spectrometry techniques have introduced completely non-invasive breathprinting; a method to instantaneously assess small metabolites in human breath. In this proof-of-principle study, we extend these findings about the impact of circadian clocks on metabolomics to exhaled breath. As previously established, our method allows for real-time analysis of a rich matrix during frequent non-invasive sampling. We sampled the breath of three healthy, non-smoking human volunteers in hourly intervals for 24 hours during total sleep deprivation, and found 111 features in the breath of all individuals, 36-49% of which showed significant circadian variation in at least one individual. Our data suggest that real-time mass spectrometric "breathprinting" has high potential to become a useful tool to understand circadian metabolism, and develop new biomarkers to easily and in real-time assess circadian clock phase and function in experimental and clinical settings.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QP Physiology | ||||||||
| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine Faculty of Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Circadian rhythms, Metabolism -- Testing | ||||||||
| Journal or Publication Title: | PLoS One | ||||||||
| Publisher: | Public Library of Science | ||||||||
| ISSN: | 1932-6203 | ||||||||
| Official Date: | 29 December 2014 | ||||||||
| Dates: |
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| Date of first compliant deposit: | 30 December 2015 | ||||||||
| Volume: | 9 | ||||||||
| Number: | 12 | ||||||||
| Number of Pages: | 16 | ||||||||
| Page Range: | pp. 1-16 | ||||||||
| Article Number: | e114422 | ||||||||
| DOI: | 10.1371/journal.pone.0114422 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access | ||||||||
| Funder: | Seventh Framework Programme (European Commission) (FP7) | ||||||||
| Grant number: | 276860 (FP7) |
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