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Phosphoinositide 3-kinase beta controls replication factor C assembly and function.
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Redondo-Munoz, J., Rodriguez, M. J., Silio, Virginia, Perez-Garcia, V., Valpuesta, J. M. and Carrera, A. C. (2013) Phosphoinositide 3-kinase beta controls replication factor C assembly and function. Nucleic Acids Research, 41 (2). pp. 855-868. doi:10.1093%2Fnar%2Fgks1095 ISSN 0305-1048.
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Official URL: http://dx.doi.org/10.1093%2Fnar%2Fgks1095
Abstract
Genomic integrity is preserved by the action of protein complexes that control DNA homeostasis. These include the sliding clamps, trimeric protein rings that are arranged around DNA by clamp loaders. Replication factor C (RFC) is the clamp loader for proliferating cell nuclear antigen, which acts on DNA replication. Other processes that require mobile contact of proteins with DNA use alternative RFC complexes that exchange RFC1 for CTF18 or RAD17. Phosphoinositide 3-kinases (PI3K) are lipid kinases that generate 3-poly-phosphorylated-phosphoinositides at the plasma membrane following receptor stimulation. The two ubiquitous isoforms, PI3Kalpha and PI3Kbeta, have been extensively studied due to their involvement in cancer and nuclear PI3Kbeta has been found to regulate DNA replication and repair, processes controlled by molecular clamps. We studied here whether PI3Kbeta directly controls the process of molecular clamps loading. We show that PI3Kbeta associated with RFC1 and RFC1-like subunits. Only when in complex with PI3Kbeta, RFC1 bound to Ran GTPase and localized to the nucleus, suggesting that PI3Kbeta regulates RFC1 nuclear import. PI3Kbeta controlled not only RFC1- and RFC-RAD17 complexes, but also RFC-CTF18, in turn affecting CTF18-mediated chromatid cohesion. PI3Kbeta thus has a general function in genomic stability by controlling the localization and function of RFC complexes.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Genomics , DNA replication | |||||||||||||||||||||
Journal or Publication Title: | Nucleic Acids Research | |||||||||||||||||||||
Publisher: | Oxford University Press | |||||||||||||||||||||
ISSN: | 0305-1048 | |||||||||||||||||||||
Official Date: | January 2013 | |||||||||||||||||||||
Dates: |
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Volume: | 41 | |||||||||||||||||||||
Number: | 2 | |||||||||||||||||||||
Page Range: | pp. 855-868 | |||||||||||||||||||||
DOI: | 10.1093%2Fnar%2Fgks1095 | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
Funder: | Madrid. Municipal government | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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