Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Corticotropin-releasing hormone intereacts with interleukin-1 beta to regulate prostaglandin H synthase-2 expression in human myometrium during pregnancy and labor

Tools
- Tools
+ Tools

Markovic, Danijela, Bari, Muhammad F., Lu, Buyu, Vatish, Manu and Grammatopoulos, Dimitris (2013) Corticotropin-releasing hormone intereacts with interleukin-1 beta to regulate prostaglandin H synthase-2 expression in human myometrium during pregnancy and labor. Journal of Clinical Endocrinology & Metabolism , 98 (7). pp. 2864-2875.

Research output not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1210/jc.2013-1094

Request Changes to record.

Abstract

Context:

The onset of labor appears to involve the activation of myometrial inflammatory pathways, and transcription factors such as nuclear factor-κB (NF-κB) control expression of the contraction-associated proteins required to induce a procontractile phenotype. These responses might involve CRH, which integrates immune and neuroendocrine systems.

Objectives:

In human myometrium we investigated cyclooxygenase 2 (PGHS2) expression and regulation by CRH and the proinflammatory cytokine IL-1β before and after labor.

Design:

Myometrial tissues obtained from pregnant women at term before (n = 12) or during labor (n = 10) and pathological cases of choriamnionitis-associated term labor (n = 5) were used to isolate primary myocytes and investigate in vitro, CRH effects on basal and IL-1β regulated p65 activation and PGHS2 expression.

Results:

In nonlaboring myometrial cells, CRH was unable to induce NF-κB nuclear translocation; however, it altered the temporal dynamics of IL-1β-driven NF-κB nuclear entry by initially delaying entry and subsequently prolonging retention. These CRH-R1-driven effects were associated with a modest inhibitory action in the early phase (within 2 hours) of IL-1β stimulated PGHS2 mRNA expression, whereas prolonged stimulation for 6-18 hours augmented the IL-1β effects. The early-phase effect required intact protein kinase A activity and was diminished after the onset of labor. The presence of chorioamnionitis led to exaggerated PGHS2 mRNA responses to IL-1β but diminished effects of CRH.

Conclusions:

CRH is involved in the inflammatory regulation of PGHS2 expression before and during labor; these actions might be important in priming and preparing the myometrium for labor and cellular adaptive responses to inflammatory mediators.

Item Type: Journal Article
Subjects: R Medicine > RC Internal medicine
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016)
Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Myometrium, Pregnancy -- Complications
Journal or Publication Title: Journal of Clinical Endocrinology & Metabolism
Publisher: Endocrine Society
ISSN: 0021-972x
Official Date: July 2013
Dates:
DateEvent
July 2013Published
10 May 2013Available
30 April 2013Accepted
10 January 2013Submitted
Volume: 98
Number: 7
Number of Pages: 12
Page Range: pp. 2864-2875
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
Funder: Wellcome Trust (London, England), Birmingham Science City

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us