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Expression of epigenetic effectors in decidualizing human endometrial stromal cells
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Grimaldi, Giulia, Christian, Mark, Quenby, Siobhan and Brosens, Jan J. (2012) Expression of epigenetic effectors in decidualizing human endometrial stromal cells. Molecular Human Reproduction, Volume 18 (Number 9). pp. 451-458. doi:10.1093/molehr/gas020 ISSN 1360-9947.
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Official URL: http://dx.doi.org/10.1093/molehr/gas020
Abstract
Cyclic differentiation of human endometrial stromal cells (HESCs) into decidual cells is a highly coordinated process essential for embryo implantation and pregnancy. This differentiation process is closely recapitulated in culture upon exposure of purified HESCs to cyclic AMP and progesterone signaling. Mining of gene expression data revealed that HESCs express 147 genes coding for epigenetic effectors, 33 (22%) of which are significantly regulated (P < 0.05) upon decidualization. Among these are genes encoding for histone-modifying proteins and their cofactors, histone-binding proteins, histone variants, CpG-binding proteins and DNA methyltransferases (DNMTs). Interestingly, more than two-thirds of differentially expressed chromatin-modifying genes are down-regulated upon the transition from a proliferative to a differentiated HESC phenotype. Despite the strong regulation of DNMTs, colorimetric and long interspersed nuclear element 1 methylation assays did not show global changes in DNA methylation levels upon differentiation of HESCs. Taken together, the coordinated regulation of diverse effector molecules suggests that complex epigenetic modification at specific loci underpins the acquisition of a decidual endometrial phenotype.
| Item Type: | Journal Article | ||||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Reproductive Health ( - until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Molecular Human Reproduction | ||||||||
| Publisher: | Oxford University Press | ||||||||
| ISSN: | 1360-9947 | ||||||||
| Official Date: | September 2012 | ||||||||
| Dates: |
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| Volume: | Volume 18 | ||||||||
| Number: | Number 9 | ||||||||
| Page Range: | pp. 451-458 | ||||||||
| DOI: | 10.1093/molehr/gas020 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access |
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